20 Frances Carter, Marion C. Woods and Miriam E. Simpson 



contained 4 to 1.5% ICSH contamination, were more variable in promoting 

 ovulation and were improved by an increase of ICSH content to 10% during 

 the preparatory phase of follicular growth. 



A much higher content of ICSH than 10% did not interfere with the 

 effectiveness of the FSH preparations either during the preparatory or 

 supplementary phases. Combinations of FSH with 4-fold as much ICSH 

 were active, optimally at doses of 2 RU FSH + 8 RU ICSH. After stimulation 

 of follicles by such combinations ovulation could be induced not only by the 

 combination but also by either component given alone as the supplement. 

 Purified FSH was somewhat more effective as a supplement and could be 

 used at lower doses than ICSH (or HCG). It was clear, however, that FSH, 

 though effective as a supplement, was sufficiently purified to require additional 

 ICSH, more than 4%, during preparation of follicles. 



It should be noted that the sheep preparations, at stages of purification 

 after the 40% ethanol starting material, contained negligible amounts of 

 other pituitary tropic hormones (growth, thyrotropic, adrenocorticotropic and 

 lactogenic hormones) so that it appears improbable that the other pituitary 

 tropic hormones are involved in either the preparatory or the supplementary 

 impetus to ovulation. Perhaps it will be impossible to clarify further the 

 proportion of the pituitary gonadotropic factors necessary for ovulation 

 until FSH and ICSH have been prepared in pure form. 



REFERENCES 



1. Bahn, R. C, N. Lorenz, W. A. Bennett and A. Albert, Gonadotropins of the 



pituitary gland and urine of the adult human male, Proc. Soc. Exptl. Biol, and Med. 

 82, 111-1%2, 1953. 



2. Carter, F., M. E. Simpson and H. M. Evans, Conditions necessary for the induction 



of ovulation in hypophysectomized rats, Anat. Record {Proceedings) 130, 283, 1958. 



3. Ellis, S., A scheme for the separation of pituitary proteins, J. Biol. Chem. 233, 63-68, 



1958. 



4. Evans, H. M., M. E. Simpson, S. Tolksdorf and H. Jensen, Biological studies of the 



gonadotropic principles in sheep pituitary substance, Endocrinology 25, 529-546, 1939. 



5. Everett, J. W., The time of release of ovulating hormone from the rat hypophysis, 



Endocrinology 59, 580-585, 1956. 



6. Fraenkel-Conrat, H. L., M. E. Simpson and H. M. Evans, Purification of follicle- 



stimulating hormone (FSH) of the anterior pituitary, Proc. Soc. Exptl. Biol, and Med. 

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7. Fraenkel-Conrat, H. L., M. E. Simpson and H. M. Evans, Purification of follicle- 



stimulating hormone of the anterior pituitary, Anales de la Facultad de Medicina, 

 Montevideo 25, 159-168, 1940. 



8. Gemzell, C. a., E. Diczfalusy and K. G. Tillinger, Clinical effect of human pituitary 



follicle-stimulatinghormone(FSH),y.C/m.£'«f/ocr/«o/.o«JMc/a6. 18, 1333-1348, 1958. 

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9. Hammond, J., Jr., Induced ovulation and heat in anoestrous sheep, /. Endocrinol. 4, 



169-180, 1945. 

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