28 



R. W. NoYES, T. H. Clewe and A. M. Yamate 



VOLUME CHANGES IN MATURING FOLLICLES 



The diameter of a given follicle, as measured in the anterior chamber of the 

 eye using an eyepiece micrometer, was estimated to be accurate to within 

 about ± 0.25 mm. Tlic volumes of 1 57 follicles in 36 transplants were recorded 

 daily from the fourth through the seventh postoperative days. In 24 of the 

 transplants, both PMS and HCG were given to the recipient animal; in 6, 



I 



10X10- 



PMS 



No treatment. 

 Pregnant mare's serum. 

 ♦_Chorionic Gonadotrophin. 



Lhcc 



7 



DAY AFTER TRANSPLANTATION TO EYE 



Fig. 13. Growth of ovarian follicles in the anterior chamber of the eye as affected by 

 pregnant mare's serum and human chorionic gonadotropin injections. Each point represents 

 the average volume of all of the grossly visible follicles in many transplants. The measure- 

 ments were made on living follicles. The points that pertain to a group of follicles that 

 first appeared on the same day are connected by lines. The time of injection and dosage 

 of PMS and HCG are given in the text. 



only PMS was given; and in 6 no gonadotropin was given. The average 

 volume for each group of follicles that appeared on a given day in each of 

 the three groups of animals was plotted against time (Fig. 13). The average 

 volume of 65 follicles that appeared on the fourth day was 40xlOV^ 

 and one day later, following castration and PMS administration, the average 

 volume had increased to 1 65 x 1 O*' /x^. When the recipients were castrated, 

 but no PMS was given, the average volume was slightly less, 120 x lO'^/x^ 

 and although they continued to grow, the follicles in uninjected animals grew 

 more slowly than those in the treated animals. 



