Follicular Development, Ovular Maturation, Ovulation in Ovarian Tissue 29 



When HCG is given there is a further increase in the follicular growth rate. 

 The curve of growth is very similar for each group of follicles irrespective of 

 the day on which they appeared. In the group of animals treated with PMS, 

 the average volume of follicles that first appeared on the sixth day is much 

 larger than that in the preceding groups. This artifact is caused by the sudden 

 emergence into view of older follicles that had been growing deeply on the 



t.oooxio- 



I 



I 

 I 



100X(0- 



4H 



^Prtgnant mam's serum, Ovary in situ. _ 

 £i " " " , Ovary in eye. 



♦ Chorionic Gonadotrophin, Ovary in situ. 

 " " , Ovary In eye. 

 X Ovary in Si+0, Adulf rat (Boling,ef:a/.)' 



• No treatment, Ovary in situ. ■ 

 o " " , Ovary in cyg- 



10X10- 



7 DAY AFTER 

 TRANSFER TO EVE 



AGE OF RAT 

 (days) 



Fig. 14. The effect of gonadotropin injections on the volumes of ovarian follicles in eye 

 transplants and in noimal immature ovaries in situ. Each point represents the average 

 volume of many follicles from different ovaries. The measurements were made on fixed, 

 sectioned tissue. The time of injection and dosage of PMS and HCG are given in the text. 



interface between the iris and the ovary. Although they appear on the sixth 

 day many of these follicles are actually older, and thus larger, than the more 

 superficial follicles. These observations vv'ere not continued long enough to 

 include the dechning growth of follicles as they become atretic, or the curves 

 for those that formed corpora lutea. 



Similar data were plotted from the average volumes of the larger follicles 

 in the serially sectioned transplants (Fig. 14). Again, three groups of trans- 

 plants were studied (castration only, PMS only, and PMS plus HCG), and 

 a parallel series of three groups of normal immature rat ovaries in situ was 

 studied for comparison. 



