Follicular Development, Ovular Maturation, Ovulation in Ovarian Tissue 31 



Approximately 6 follicular ova were pipetted into each of 184 ovarian 

 bursas of previously mated albino recipient animals. The method for injecting 

 ova is illustrated in Fig. 15. 



In a previous experiment (1), when 130 developing follicular ova were 

 removed from the ovaries of normal adult animals six hours or less before 

 the expected time of ovulation, and were then transferred into the bursas of 

 19 recipient animals, 44 (34%) of the ova were fertilized and developed to 

 term embryos. 





"JT" 



® No. Q\ia in iQcb transfap 

 El No. all OvQ +rans-fcrr4d 



6 6 10 12 H 15 16 17 18 19 20 Zl 22 27 24 



NO. HM. ELAPSED FOLLOWING CHORIONIC GONADOTROPHIN INJECTION 



Fig. 16. The fertility of follicular rat ova taken from ovaries transplanted to the eyes of 

 recipient rats of a different strain. The data are from Table I . The figures within the circles 

 are the numbers of ova in each transfer in which one or more of the transferred ova 

 developed to term. The figures in the squares represent the number of all ova transferred 

 into recipient bursas at a given hour following chorionic gonadotropin injection. 



Our present experience with the fertility of follicular ova obtained from eye 

 transplants is not nearly this encouraging (Table 1). When 809 maturing ova 

 from eye transplants were transferred to 131 bursas, only 36 (4.5 %) developed 

 into term embryos. 



The optimal stage of folHcular development was between the fourteenth 

 and sixteenth hours following the administration of HCG (Fig. 16), but even 

 at this time only 10% of transferred ova survived. The results of individual 

 experiments were quite variable, and the apparent high fertility of ova 

 occurring 24 hr after HCG was probably a chance occurrence. This was a 

 very rigorous test for fertility, with many chances for ova to be lost and for 

 inadequate conditions for fertilization to be present. However, the conditions 

 in these eye transplant experiments were similar to those with normal 

 follicular ova, yet only one-tenth as many of the ova from the eye transplants 

 were fertile as compared with the preovulatory ova from ovarian follicles 

 in situ. 



