72 G. W. Harris 



eminence extracts infused in the pituitary were efTective in evoking ovulation 

 (except in the case of the two animals that received the smallest dose), 

 whereas control infusions of the cerebral cortex in the pituitary failed to 

 excite the same response. Intravenous infusion of median eminence extract 

 was likewise ineffective in causing ovulation except in one animal that was 

 exceptional in that it was injected with the maximal dose (5.4 mg of extract) 

 over a period oi\)ne minute. Thirteen animals in which no infusion was given 

 failed to ovulate after nembutal treatment. 



The preliminary results presented above dealing with the infusion of 

 median eminence extracts into the pituitary glands of rabbits and rats are 

 suggestive, but further work is necessary before any definite conclusions 

 can be drawn. 



Acknowledgments — The original work reported in this paper (by H. J. 

 Campbell, G. Feuer, J. Garcia and G. W. Harris) was performed with the 

 assistance of a grant from the United States Air Force (Contract No. AF61 

 (514)-953) and (by M. Nikitovitch- Winer) a postdoctoral Fellowship Award 

 from United States National Institutes of Health (AF-8155-CI). 



REFERENCES 



1. AsDELL, S. A., Studies in the physiology of lactation, Dissertation, Cambridge 



University, England, 1926. 



2. Baker, J. R. and R. M. Ranson, Proc. Roy. Soc. S 110, 313, 1932. 



3. Bauer, H. G., /. din. Endocrinol. 14, 13, 1954. 



4. Benoit, J. and I. Assenmacher, Arch. Anat. micr. 42, 334, 1953. 



5. Benoit, J. and I. Assenmacher, /. Physiol. (Paris) 47, 427, 1955. 



6. Benson, G. K. and S. J. Folley, J. Endocrinol. 16, 189, 1957. 



7. Bissonnette, T. H., Proc. Roy. Soc. B 110, 322, 1932. 



8. Bissonnette, T. H., Res. Publ. Ass. nerv. ment. Dis. 17, 361, 1938. 



9. Bogdanove, E. M. and H. C. Schgen, Proc. Soc. exp. Biol., N.Y. 100, 664, 1959. 



10. Brooks, C. McC, Amer. J. Physiol. 113, 18 P, 1935. 



11. Brooks, C. McC, Amer. J. Physiol. 120, 544, 1937. 



12. Brooks, C. McC, Amer. J. Physiol. 121, 157, 1938. 



13. Byrnes, W. W. and R. K. Meyer, Endocrinology 48, 133, 1951. 



14. Campbell, H. J. and G. W. Harris, /. Physiol. 136, 333, 1957. 



15. Clark, H. M., Anat. Rec. 61, 175, 1935. 



16. Clark, W. E. Le Gros, T. McKeown and S. Zuckerman, Proc. Roy. Soc. B 126, 



449, 1939. 



17. DoHRN M. and W. Hohlweg, Proc. 11 Int. Congr., Sex Res., pp. 436^442, London, 1931. 



18. Donovan, B. T. and G. W. Harris, /. Phvsiol. 131, 102, 1956. 



19. Donovan, B. T. and G. W. Harris, J. Physiol. 132, 577, 1956. 



20. Donovan, B. T. and G. W. Harris, in Marshall's Physiology of Reproduction, Vol. I, 



Part 2 (Edited by A. S. Parkes) (in press). Longmans, Green & Co., London, 1960. 



21. Donovan, B. T. and J. J. van der Werff ten Bosch, Nature, Lond. 178, 745, 1956. 



22. Donovan, B. T. and J. J. van der Werff ten Bosch, J. Physiol. 132, 57P, 1956. 



23. Donovan, B. T. and J. J. van der Werff ten Bosch, J. Physiol. 147, 78, 1959. 



24. Donovan, B. T. and J. J. van der Werff ten Bosch, J. Physiol. 147, 93, 1959. 



25. Eckstein, P. and S. Zuckerman, in Marshall's Physiology of Reproduction, 3rd Ed., 



Vol. I, Part 1 (Edited by A. S. Parkes), p. 257, Longmans, Green &. Co., London, 1956. 



