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Discussions 



controlling gonadotropin release. In addition, a complete analysis of this experimental 

 situation provides good evidence for the action of steroids on neural tissue, directly. 

 The Japanese workers, Takewaki and Tagasuki, have shown that the steroid-induced 

 sterility follows the post-natal administration of corticoids and estrogens, as well as 

 androgens. PfoitTcr (Amer. J. Amit. 58, 195-225, 1936) described the same result with 

 the implantation of new-born testes in litter-mate females. 



The need for pre-treatment with progesterone to cause electro-stimulated ovulation 

 in these anovulatory animals has been interpreted by Dr. Barraclough to indicate 

 that without progesterone the anovulatory female pituitary is deficient in stored LH. 

 We have assayed these gonadotropins with respect to both total gonadotropins, based 



Table 1. Gonadotropin Content of Pituitary Glands 



* Averages ± standard deviation calculated from the weights of 20 pituitary glands 

 of each type. 



t Assay animals were immature female rats; control ovaries average 32 mg. All weights 

 represent average of 3 pairs of ovaries following a three-day injection period. 



Table 2. Three Months Mating Record of Anovulatory Rats 



* Pregnancy-type of vaginal smear, 13-22 days' duration. 



t Diestrus smear, 3-5 days' duration, followed by continuous vaginal comification. 



J Constant vaginal comification. 



§ Corpora lutea. 



