Induction of Ovulation in the Human by Human Pituitary Gonadotropin 209 



amounts of LH were added did the follicles produce and release estrogen. 

 As the human pituitary FSH preparation contained small amounts of LH 

 it was impossible to draw any conclusions on this subject. The problem will 

 not be solved until a pure human pituitary FSH preparation is available and 

 is injected into hypophysectomized individuals. 



The response to human pituitary FSH was different in amenorrheic women 

 with atrophic endometrium indicating no ovarian activity and in those who 

 have proliferative endometrial activity indicating some ovarian function 

 (Table 2). None of the women with atrophic endometrium ovulated following 

 the administration of FSH alone while 7 out of 10 with proliferative activity 

 ovulated. 



It seems most likely to assume that the difference is at the pituitary level. 

 The pituitaries of the first gioup of women lacked the capacity to release 

 LH when stimulated by estrogen while the pituitaries of the second group 

 released enough LH for an ovulation to take place (Fig. 12). It might also be 

 suggested that the two amenorrheic women who ovulated on the administra- 

 tion of FSH had pituitaries which were insufficient in LH. One of them 

 ovulated repeatedly during one year following the administration of HCG. 



Acknowledgement. — This work has been done in collaboration with Dr. E. 

 Diczfalusy and Dr. K.-G. Tillinger, Karolinska Hospital, Stockholm. 



REFERENCES 



1. WiTSCHi, E. and C. Y. Chang, Symposium on Comparative Endocrinology, John Wiley, 



1959. 



2. VAN Wagenen, G. and M. E. Simpson, Endocrinology 61, 316, 1957. 



3. Li, C. H. and H. Papkoff, Science 124, 1293, 1956. 



4. Gemzell, C. a., E. Diczfalusy and K. G. Tillinger, J. Clin. Endocrinol. 18, 1333, 



1958. 



5. Brown, J. B., Biochem. J. 60, 185, 1955. 



6. Diczfalusy, E. and A. Westman, Acta endocrinol. 21, 321, 1956. 



7. Brown, J. B., R. D. Bulbrook and F. C. Greenwood, /. Endocrinol. 16, 49, 1957. 



8. Klopper, a., E. a. Michie and J. B. Brown, /. Endocrinol. 12, 209, 1955. 



9. Allen, W. M., J. Clin. Endocrinol. 10, 71, 1950. 



10. Diczfalusy, E., Acta endocrinol. 20, 216, 1955. 



11. Gemzell, C. A., E. Diczfalusy and K. G. Tillinger, Acta Obst. et Gyn. Scandinav. 



38, 465, 1959. 



