(;4 



imlriciit media, in lesl liilx's. Xoiic of llic moulds were 

 injured by a ^-liour sul)e()olini;' at -14°. AspcrfjiUus sur- 

 vived subeoo]iiii>' for 4 days at - G° to -11°. Increased 

 concentration of tlie medium resulted in increased cold 

 resistance of liie suspended fuiis;i. In the frozen condi- 

 tion, ill 1 i)er cent <>hicose solution, AspergiUus was killed 

 after 2 hours at -12°, while in 50 per cent glucose, no 

 injury was apparent after 2 hours at -26°. The other 

 three fungi gave similar results, though their resistance 

 was not (piite so high. In genei-al, the resistance of the 

 hyphae increased with age. 



Bartram (1916) exposed a number of fungi, cultivated 

 on agar slants of varying composition, to the cold of winter 

 for 4 months during which time a minimum temperature 

 of - 29° was reached. All the Sderotinia, Ceplialothecium, 

 Glomerella, Ventnria, and Ascophyta survived, while 

 Alteniaria, Cijlindrosporium, Plowriglitia and Pliifto- 

 ph tJwra survived on some media but not on others. Sphae- 

 ropsis and Fusarhnn were killed. 



According to Richter (1910), the mycelia of Aspergillus 

 niger, cultivated in liquid nutrient media, survived a 24- 

 hour exposure to -10° to -13°. In another series of 

 experiments, he exposed the material to - 12° for two 

 days, then allowed it to grow for 3 days at + 30°, there- 

 upon exposed it to about -80° for 12 hours, again kept it 

 at ^ 30° for 24 hours, and finally exposed it over night to 

 -11° and let it thaw rapidly. No injurious effect of the 

 treatment could be observed. 



Chodat (1896) exposed for 4 hours to temperatures of 

 - 70° to - 110° Mucor mycelium growing on agar or on 

 liquid medium. After thawing, the filaments seemed to 

 be dead, but some growth originated from them later. A 

 part of the mycelium which was entirely formed within 

 the liquid medium, showed, after thawing, a tendency to 

 roll into a ball as dead mycelium does, but, after a few 

 days, new growth originated from it. 



ileldmaier {Zfschr. /. Bof., 22, 170, 1929) subjected the 

 mycelia of Schizophylluui and CoUyhia, cultured on agar 



