180 EXPERIMENT STATION EECOED. 



per cent). The reason that varient results were obtained with the optical 

 method lies principally in the inability of the sera to produce a uniform cleav- 

 age of the peptone at different times, since some protective ferments will cleave 

 ft certain kind of peptone while others will attack this peptone only slightly. 

 In the dialysis method, dialysis thimbles were used. The method was controlled 

 by the optical procedure 92 times and conducted 7 times alone. Agreement was 

 noted in 61 cases, 43 positive and 18 negative, showing that the results from 31 

 cases did not harmonize. A very high cleavage power was noted with the sera 

 of eclamptic patients. 



Experiences with the Abderhalden pregnancy reaction (dialysis procedure 

 and ninhydrin reaction), H. Schlimpert and J. Hendey {Mmwhen. Med. 

 Wclmschr., 60 (1913), No. 13, pp. 681-685; abs. in Chem. Abs., 7 {1913), No. 13, 

 p. 2242). — The findings of Abderhalden were verified in 79 cases, of which 40 

 were absolute cases of pregnancy. In all cases of pregnancy the reaction was 

 positive and the rest of the sera gave negative reactions. 



"A total of 316 cases were examined but not with satisfactory results until all 

 the important points in the technique had been worked out. Especially sig- 

 raficant was the fact that placenta not blood-free gave inaccurate results. For 

 washing the placenta tissue sodium chlorid below 0.5 per cent proved unde- 

 sirable ; above this concentration to 0.9 per cent the blood was readily removed 

 and clear white placenta tissue was obtained. The procedure finally adopted 

 was to wash out the placenta tissue with 0.9 per cent sodium chlorid until 

 colorless, then wash 10 minutes in running tap water to remove the sodium 

 chlorid, and proceed further as directed by Abderhalden. Other less important 

 details of the technique are discussed." 



Abderhalden's reaction with placenta and serum, H. Schlimpert and E. 

 IssEL {Miinchen. Med. Wchnschr., 60 (1913), No. 32, pp. 1758-1760; abs. in 

 Berlin. Tierarztl. Wclmschr., 29 (1913), No. 37, p. 665).— The tests were con- 

 ducted with the object of determining whether animals during the period of 

 pregnancy developed certain ferments and to elicit to what extent the ferments 

 are specie specific. It was considered of great importance in the investigations 

 to separate sharply the fetal and maternal parts of the placenta in order to get 

 a substrat which was subject to better control. From theoretical considera- 

 tions it was also deduced that a migration of chorion villi in the blood stream 

 of the mother is without reasonable foundation. For the tests sheep and horse 

 placentas were used. 



Ferments were noted in the blood stream which were not only specific for 

 homologous placenta protein but also for the heterologous protein, e. g., the 

 strongest serum was from man and its action was stronger for horse protein 

 than for human placenta protein. Sheep serum also acted more strongly on 

 horse protein than on sheep placenta protein. The fetal as well as the maternal 

 parts of the placenta were cleaved. 



Serology of eclampsia, B. Stance (Zenthl. Gyndkol., 37 {1913), No. 9, pp. 

 298-300; ahs. in Jour. Amer. Med. Assoc, 60 {1913), No. U, p. iii7).— Abder- 

 halden's reaction for pregnancy was found especially intense with an eclamptic 

 placenta obtained from a woman. The changes in the blood in eclampsia are 

 similar to those of a normal pregnancy. 



Abderhalden's dialysis method, a means of determining whether or not 

 a cow is in a new period of lactation, H. Falk {Berlin. TierdrzU. Wchnschr., 

 29 {1913), No. 8, pp. 129-132) .—ThQ author gives his experience with the 

 Abderhalden dialysis method (E. S. R., 28, p. 777), which was previously pro- 

 posed for diagnosing pregnancy. It was found that the blood contained pro- 

 teolytic enzyms 21 days after calving, and in some cases on the twenty-third 



