378 EXPERIMENT STATION RECORD. 



veiu of a horse or other domestic auiiual is drawn into a flask containing a 

 sufficient quantity of 1 per cent sodium citrate solution to prevent coagulation. 

 It is then centrifuged, the sodium citrate solution and serum drawn off, and 

 the corpuscles treated with 0.5 per cent acetic acid solution. It is then cen- 

 trifuged again and the process repeated several times with acetic acid until 

 the mass of corpuscles is free from red blood corpuscles. 



" The leucocytes are then washed with physiologic salt solution 3 or 4 times 

 to remove most if not all trace of the acetic acid, after which they are ground 

 in a mortar with quartz sand. To the washed and ground leucocytes is added 

 about 5 volumes of sterile distilled water to 1 volume of leucocytes. This mix- 

 ture is exposed to a temperature of 58° C. for 1 hour, when it is placed in the 

 incubator for a period of 12 hours, and again exposed to a temperature of 58° 

 for one hour. This process of exposure to different temperatures is continued 

 for 2 or 3 days or until autodigestion is complete. It is then centrifuged, the 

 supernatant fluid decanted, and sufficient tricresol added for preservative pur- 

 poses." The extract so prepared, when given parenterally, gives rise to a 

 marked leucocytosis. 



" While the total leucocyte counts were intensely interesting, the differential 

 counts were infinitely more so. The polymorphonuclear neutrophils increased 

 about 100 per cent, the small mononuclears decreased about 100 per cent, the 

 large mononuclears decreased about 25 per cent, while the eosinophils in- 

 creased about 800 per cent. In all our experiments we found that there oc- 

 curred a marked eosinophilia. In fact, in one rabbit the eosinophils showed 

 by differential count 44S per cent of the total count." 



" The practical therapeutic application of leucocytic extracts has been fol- 

 lowed out during the past year, not only in our own practice, but also by other 

 practitioners. Various infections have been treated with, in some types of in- 

 fections, encouraging results, while in others the beneficial results were not so 

 marked. 



"The diseases to which the treatment has been applied, and which have 

 given the most gratifying results are such infections as purpura, influenza, 

 pneumonia, distemper in dogs, etc." 



Autogenous vaccines, W. W. Lang {Vet. Rec, 26 {1914), ^'o. 1331, pp. 434, 

 ^35), — In this brief article the author relates his experiences with the use of 

 autogenous vaccines, especially for cases of strangles and poll evil. Their use 

 is recommended. 



Experiences with the Abderhalden dialysis method. — I, Cleavage of 

 thymus tissue by a normal serum, Helene Deutsch {Wiener Klin. Wchmchr., 

 26 {1913), No. 38, pp. 1492-1494; abs. in ZentU. Expt. Med., 4 {1913), No. 12, 

 p. 5^0). — Thymus-cleaving ferments were noted in 51 out of 55 sera coming from 

 healthy humans varying in age from 4 to 70 years. 



Observations on the protective enzyms of the body (Abderhalden), E. G. 

 Grey {Bui. Johns Uopkins Hosp., 25 {1914), No. 278, pp. 117-122).— This reports 

 the results of a study of the Abderhalden (protective enzym) method, with a 

 view to ascertaining the relationship of these enzyms to epithelial transplants. 

 The ferments are considered specific. 



Diagnosis of malignant new formations and pregnancy with the Abder- 

 halden method, G. von Gambaroff {Miinchen. Med. Wchnschr., 60 {1913), No. 

 30, p. 1644; a^s. in ZentU. Expt. Med., 5 {1914), No. 1, p. 17).— In only 3 out of 

 50 cases of tumor were the findings negative. Serum from cases of carcinoma 

 cleaves carcinomatous tissue but not sarcoma tissue. The inverse holds good 

 also. 



Experimental contribution to the diagnosis of pregnancy by Abder- 

 halden's dialysis method. Naumann {Deut. Med. Wchnschr., 39 {1913), No. 43, 



