580 EXPEEIMENT STATION EECORD. 



In four different instances in cultures from the passage and fixed virus nucle- 

 ated round or oval bodies, surrounded with a membrane totally different from 

 the minute bodies mentioned above, were noted. 



" By inoculating cultures containing the granular, pleomorphic, or nucleated 

 bodies, rabies has been reproduced in dogs, rabbits, and guinea pigs, as shown 

 by the typical symptoms and positive animal inoculations, while the film prepa- 

 rations from the brain of the animals contained always the granular and some- 

 times the nucleated bodies in large numbers." 



Contribution to the study of the fi.ltrate of the rabies virus, Neumann and 

 T. MiRONESCO {Compt. Rend. Soc. Biol. [Paris], 74 (1913), No. 12, pp. 112, 

 713). — The filtrate from the rabies virus has been found to be very variable as 

 regards strength. In order to see if a homogeneous virus could be obtained, 

 the brain of a rabbit which died of the disease was rubbed up in a mortar with 

 100 cc. of a normal serum, and the emulsion obtained was shaken up in a bottle 

 containing glass beads for about one hour. The emulsion was then filtered 

 through a sterile gauze, and at this juncture there was added an emulsion made 

 from two 24-hour agar cultures of the Bacillus pyocyaneus and emulsified with 

 normal serum. The entire mixture was then diluted with 300 cc. of normal 

 serum, mixed well, and filtered through a Berliefeld V filter under a pressure 

 of from two to five atmospheres. 



Some rabbits were then inoculated with the filtrate and others with the 

 unflltered virus. The rabbits receiving the filtrate died in the same time as 

 those receiving the unfiltered virus. The brains of the rabbits dying as a 

 result of the injection of the filtrate were infectious for other rabbits. 



The diagnosis of rabies, H. Koenigsfeld (Centhl. Balct. [etc.], 1. AM., Orig., 

 70 (1913), No. 1-2, pp. 85-98; abs. in Ztschr. Fleisch u. Milchhyg., 24 {1913), 

 No. 3, p. 64)- — In this work it was possible to verify the findings of others 

 that the rabies virus can penetrate the skin or mucous membrane through super- 

 ficial wounds and cause the disease. The corneal and cutaneous methods will in 

 many cases determine the presence of the disease even though putrefied mate- 

 rial is used for the tests. The incubation periods, etc.. ai'e often lengthened 

 when putrefied material is used in the experiments. 



The treatment of tetanus, J. Schmidt (Bcr. K. Tierdrztl. Hochschule Dres- 

 den, n. scr., 7 (1912), p. 151; al)s. in Vet. Rec, 26 {1913), No. 1324, P- 326).— 

 A comparison of nine cases of tetanus in the horse. Two of the animals were 

 treated with morphin and chloral hydrate alone and the other seven with 

 tetanus antitoxin only. The two first-mentioned horses were slaughtered, being 

 incurable, one after 12 hours and the other 3i days after treatment. Of the 

 other seven, four recovered, two died, and one was slaughtered. The anti- 

 toxin was given intravenously. 



Double infection with the human and bovine types of Bacillus tuberculo- 

 sis, P. A. Lewis {Jour. Amer. Med. Assoc, 60 {1913), No. 3, pp. 202, 203).— 

 A description of a case in a man, by occupation a butcher, in which the lungs 

 were affected by the human type of organism, and the thumb (tuberculosis 

 verrucosa cutis) by the bovine type of organism. 



Investig'ations in regard to the isolation of the human and bovine types 

 of tubercle bacilli from cultures of atypical virulence (Schroeder-Mietzsch 

 strain) and from artificially mixed cultures, E. A. Lindemann (Arb. K. 

 Osndhtsamt., 45 {1913), No. 2, pp. 197-225) .—The Schroeder-Mietzsch culture 

 is an atypical culture isolated from a 20-year old woman affected with pul- 

 monary tuberculosis. It grows like the bovine type in bouillon, but lacks the 

 virulence of this type of organism. Thinking that it might be a mixed culture, 

 although investigations by others have proved the contrary, the author made 

 tests with it on rabbits, guinea pigs, and chickens, and in bouillon. The i-esults 



