VETEBINARV MEDICINE. 777 



insect or in the fecal deposits. The virulence of the organisms present was 

 demonstrated by the results with the animals inoculated from suspensions of 

 agar cultures, there being a uniform fatality from the material used begin- 

 ning with the three hours up to and including nine days. " Beginning with the 

 cultures of feces made after the ninth day following the infective meal, all 

 proved avirulent as far as animal inoculation was concerned. All of the ordi- 

 nary cultural and morphological tests were positive from the ninth day to the 

 twentieth day with tlie material obtained from S. calcitrans, and from the 

 sixth to the tenth day with material obtained from T. sh'iatus." 



See also a previous note (E. S. R., 30, p. 780). 



A case of myiasis aurium accompanying' the radical mastoid operation, 

 G. M. CoATES (Jour. Amer. Med. Assoc, 63 {19 U), No. 6, pp. 479, .^80).— The 

 screw-worm {Compsomyia macellaria) is thought to have been the parasite 

 concerned. 



Collected studies on the insect transmission of Tr3rpanosoma evansi, M. B. 

 MiTzMAiN (Pub. Health Serv. V. 8., Hyg. Lab. Bui. 94 {.1914), pp. 7-39, pis. 5).— 

 This collection consists of the following articles: (1) The Relation of Tabanus 

 striatus to Suri-a Dissemination (pp. 7-19), previously noted (E. S. R., 30, p. 

 253) ; (2) The Relation of Mosquitoes to Surra (pp. 20-23) ; (3) Notes on the 

 Bionomics of Lyperosia exigua and the Relation of this Fly to Experimental 

 Trypanosomiasis (pp. 24-30) ; (4) Mechanical Transmission Experiments with 

 Philcematomyia crassirostris (pp. 31, 32) ; (5) Experiments with Hippobosca 

 maculata in the Transmission of Surra (pp. 33, 34) ; (6) Experiments with 

 Bloodsucking Gnats (p. 35) : and (7) The R51e of Musca domestica in Surra 

 Conveyance (pp. 36-39). 



A new staining method for preparing granulated tubercle bacilli, T. 

 ISHiWARA (Centbl. Bakt. [cte.], 1. Abt., Orig., 68 {1913), No. 1, pp. 113-117).— 

 The following method is recommended for this purpose : 



(a) Stain for 2 minutes with a petroleum ether water-fuchsin solution pre- 

 pared by filling the conical portion of a test tube with petroleum ether, adding 

 sufficient distilled water to fill three-fourths of the tube, filtering through mois- 

 tened filter paper, and add one-fourth of the volume of carbol-fuchsin solution 

 (100 cc. of 5 per cent carbolic acid and 10 cc. of a saturated solution of fuchsin) ; 

 (b) decolorize for 2 minutes in a 25 per cent solution of nitric acid and follow 

 by rinsing with 70 per cent alcohol until the preparation appears colorless; (c) 

 stain with a saturated aqueous solution of methylene blue. After staining 

 wash well with water to prtA-ent a precipitation of the coloring matter. 



The modified Gram staining method was also simplified by utilizing a petro- 

 leum ether water-carbol-gentian violet solution. In addition to this a simpli- 

 fied method for staining the Much granules and the granular form of the 

 tubercle bacillus is described. 



About the occurrence of tubercle bacilli in apparently healthy mammary 

 gland tissues of cows destined for slaughter, T. Ishiwara {Centbl. Bakt. 

 letc.], 1. Abt., Orig., 70 {1913), No. 1-2, pp. i-iO).— Mammary glands macro- 

 scopically free from tubercular changes but from cows having organs affected 

 with tuberculosis were found in 5 out of 26 cases to contain tubercle bacilli. 

 Most of the udders came from animals which had the severest form of the 

 disease. 



Study of the pathological changes which occur in the organs of tubercular 

 pigs and the detection of granulated tubercle bacilli, T. Ishiwaea (Berlin. 

 TierdrztL Wchnschr., 29 (1913), No. 37, pp. 657, 658).— In this work the organs 

 of 217 pigs were examined for the presence of tubercle bacilli. The lungs were 

 tubercular in 181 instances, the bronchial lymph glands in 173, the mesenteric 

 lymph nodes in 147, the spleen in 132, the tracheal nodes in 126, the liver in 



