778 EXPERIMENT STATION EECOED. 



83, the shoulder glands In 82, the kidneys in 55, the kneefold nodes in 21, the 

 bones in 12, the joints in 8, and the muscles in 2 cases. A table Is given which 

 shows the pathologic appearance of the various organs. 



In a previous paper the author pointed out that the tubercle bacilli present 

 in hog tuberculosis are very hard to detect, as staiuable forms are only 

 sparingly present. The presence of much fat in and about the organisms is 

 not the cause for not detecting the stain. Using Much's method it was found 

 that as a rule the granulated bacilli from the purulent and calcified areas are 

 harder to detect than the organisms present in the compact nodules and 

 caseated areas. 



Contribution to the biology of the tubercle bacillus, I, G. Lookemann 

 {Deut. Med. Wchnschr., 39 {1913), No. 50, pp. 2Jt58, 2455).— Weighing the cul- 

 tures of the tubercle bacillus from time to time is considered a good procedure 

 for obtaining a clew to the state of the growing process. Tubercle bacilli 

 cultures, after having reached their maximum of growth, lose weight, un- 

 doubtedly because of an autolytic process going on in the culture. There was 

 no difference in the rate of growth when different culture media (glycerin 

 bouillon, or asparagin solution) were used. However, the acid titer obtained 

 with the two cultures was different, and the curve with the glycerin bouillon 

 took a middle position between the titers set down for the human and bovine 

 types of organism. 



During the growth of the tubercle bacillus in asparagin solutions, 1. e., 

 protein-free media, substances were liberated which gave reactions charac- 

 teristic of the proteins. 



Serologic investigations of the antigen content of the cultures of tubercle 

 bacilli, B. Mollers {Deut. Med. Wchnschr., 39 {1913), No. 50, pp. 2460, 2461).— 

 The antigens noted by the complement fixation method in cultures of tubercle 

 bacilli increased up to the sixth week of growth. A diminution of antigen took 

 place at the eighth week. 



Theobald Smith's reaction curve as an aid for differentiating human and 

 bovine tubercle bacilli, J. Wankel {Deut. Med. Wchnschr., 39 {1913), No. 50, 

 p. 2461).— The value of this method (E, S. R., 17, p. 294) for diagnosing the 

 kind of culture in hand was investigated with 45 cultures. With 25 cultures 

 of the human type only 11 gave a characteristic Smith curve, and 6 formed no 

 acid at all but otherwise behaved like bovine bacilli. Out of 20 cultures which 

 were adjudged by the animal experiments to be of the bovine type, 15 behaved 

 as bovine cultures with the reaction curve, 4 formed acid which, according to 

 Smith would be classed with the human type, and the remaining culture could 

 not be classified. 



See also a pi'evious note by Grund (E. S. R., 26, p. 582). 



Intraperitoneal lysis of tubercle bacilli, W. H. Man waking and J. Bbon- 

 FENBKENNEB {Jouv. Expt. Mcd., 18 {1913), No. 6, pp. 601-617, figs. 4> obs. in 

 Science, n. ser., 38 {1913), No. 978, pp. 453, 454; Proc. 8oc. Expt. Biol and Med., 

 10 {1913), No. 3, pp. 67, 68). — "If suspensions of tubercle bacilli are injected 

 into the peritoneal cavities of tuberculous guinea pigs, there takes place a rapid 

 disappearance of the bacilli from the peritoneal fluids, as determined by subse- 

 quent examinations by the Ziehl-Neelsen method. Nine-tenths of the bacilli 

 may disappear within an hour, and all but an occasional bacillus within five 

 hours. This disappearance is paralleled by the appearance of atypical, nonstain- 

 ing, and granular forms. After the disappearance numerous granules can be 

 demonstrated in the peritoneal fluids and peritoneal scrapings by the Much 

 method. 



" Before the conclusion can be drawn, however, that the disappearance of 

 the tubercle bacilli Is due wholly to their destruction by the peritoneal fluids, 



