32 



MAURICE J. BESSMAN 



TAHLE XIV 



Effect of Extent of Net Synthesis on Composition 

 OF Enzymatically Synthesized DNA" 



" From Lehman et al. (1958a). 



thesis of the DNA could be influenced by lowering the concentration of 

 any one of the four deoxyribonucleoside triphosphates. 



2. Nearest Neighbor Anahjsis 



Although it is not yet possible to determine the sequence of nucleo- 

 tides in DNA, it has been possible to examine the frequency of nucleo- 

 tide pairs in products prepared from several different primers. The tech- 

 nique as developed by Josse et al. (1961) involves the following 

 operations : 



a. Synthesis of four identical products each one containing a different 

 P^--labeled dcoxynucleotide. This is accomplished by setting up incuba- 

 tion mixtures containing the following deoxyribonucleoside triphosphates: 



1. dATP32 + dTTP + dGTP + dCTP 



2. dATP -1- dTTP32 4- dGTP -|- dCTP 



3. dATP + dTTP + dGTP32 + dCTP 



4. dATP + dTTP + dGTP + dCTP^^ 



