0.6- 



Isp 



No triphosphates 



dATP 



TTP 



dCTP+dGTP 



150 250 350 



Time in minutes 



Fig. 13. Synthesis of DNA and deox^-adenylate-thymidylate copolymer, with 

 thymus DNA as primer. (From Lehman, 1959.) 



+ 0.1 

 0.0 



-0.1 

 0.2 



^0.3 



o 



CM 0.4 



d 



<J0.5 



0.6 

 0.7 

 0.8 



20 ^0 60 60 100 120 140 160 

 Time in minutes 



Fig. 14. Comparison of primed versus unprimed synthesis of d-AT copolymer. 

 (From Schachman et al., 1960.) 



42 



