CONTENTS XI 



Chapter VI 



Biosynthesis of RNA in Relation to Genetic Coding 



Problems 



Elliot Volkin 



I. Introduction 271 



II. Evidence Implicating an RNA Species as a Carrier of Genetic 



Information 273 



III. Mechanism of Messenger RNA Function Based on in Vivo 



Experiments 277 



IV. Attempts at Isolation of a Messenger RNA 279 



V. In Vitro Synthesis of RNA 280 



VI. RNA Involvement in in Vitro Protein Sj-nthesis 281 



VII. The Triplet Code 284 



VIII. Summary 286 



References 286 



C/iapfer VII 



Kinetic Studies of the Synthesis of RNA and Ribosomes 



R. B. Roberts, R. J. Britten, and B. J. McCarthy 



I. Introduction 292 



II. Properties of Ribosomes in Vitro 299 



III. Fractionation of Ribosomes 308 



IV. Metabolic Pools 311 



V. Kinetics of Incorporation into RNA of Ribosomes 315 



VI. Kinetic Studies of Phenol-Extracted RNA 329 



VII. Kinetics of Incorporation into Protein 331 



VIII. Composition of Newly Formed RNA 335 



IX. Discussion 342 



X. Epilogue 350 



References 350 



Chapter VIII 

 Protein Synthesis in Relation to Gene Action 



Richard Schweet and John Bishop 



I. Introduction 353 



II. Formation of Amino Acryl-RNA Compounds 355 



