400 KICHAHl) >('in\i:KT and ,I()11.\ UlSIlol* 



fomploto tjy^Loin or by inciil)ali()n w ilh (•liclalin^ a^fiits, witlumt aricctin^ 

 the poly-U stimulated incorporation. These results (R. Arlinghaus and 

 R. Sclnvcct. unpublished data) indicate that honiOG;lobin and poly])hcnyl- 

 ahmine are .synthesized on separate sites. 



It seems likely that a fraction similar in function to messenger HNA 

 is present in reticulocyte ribosomcs (G. Favclukes, li. Hardesty, and 

 R. Schweet. unpul)iished data). Ribosomal RNA from i-cticidocytcs 

 stimulated amino acid incorporation when added to the usual pi-ciiim- 

 bated E. coli .system. Whether this stimulation represents even a small 

 amount of globin synthesis or not, is not known. Our present conce])t, 

 tliercfore, is that the liasic mechanism of information ti'ansfci- in higher 

 organisms is similar to bacteria. However, at least in tlie reticulocyte, 

 the messenger RXA is firmly bound in the ribosome and if it is destroyed 

 and replaced at all, this is a slow process. The synthesis of messenger 

 RNA probably occurs only in the nucleus. 



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