446 



CORRADO BAGLIONI 



\)V('\\ sliuwii to have ahiionual a cliains by dissociation and recombina- 

 tion rxperinients (Ganiniack et a/.. 1960) and it has been suggested that 

 Hb-G-.. is a combination of atiiha.ian chains with 8^- chains (a2Gibadan 

 Sv^-). Keconibination of Hb-Gn.a.ian with Ilb-A,. produced some Hb-Go 

 antl Hb-A (Huehns and Shooter, 1961). 



Several reports have appeared recently in the literature of a "split" 

 Hb-Ao in individuals who possess an abnormal hemoglobin. One of the 

 minor components has the electrophoretic mobility characteristic of 

 Hb-A.., while the abnormal minor component shows the same difference 

 in electrophoretic mobility relative to Hb-A, that the abnormal major 

 component shows relative to Hb-A (Weatherall and Boyer, 1961). Bou- 

 lard et al. (1961) have fingerprinted the abnormal minor component 

 present in a Hb-I carrier and Baglioni (1962a) has fingerprinted the 



Fig. 12. Schematic e.xplanation of the presence of four hemoglobins in individuals 

 heterozygous for an a chain mutation (Baglioni, 1962a). The genotype is illustrated 

 on the left (No =: Norfolk). The arrows show the different dimers synthesized 

 under the control of the two a alleles and of the fJ and 8 genes. The dimers combine 

 randomly to form the four hemoglobins indicated by capital letters. The /? and 5 

 loci arc linked (Ceppellini, 1959h). 



abnormal minor component present in a Hb-Norfolk carrier. Both Hb-I 

 and Hb-Norfolk arc known to have abnormal a chains (Murayama and 

 Ingram, 1959; Baglioni, 1962a). The fingerprints of the abnoraial minor 

 components have shown the presence of « chains with alterations similar 

 to the one reported for Hb-I and Hb-Xorfolk, respectively, and of 

 normal S-^= chains. 



The a chains of Hb-A and of Hb-A- have been shown to be chem- 



