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QUESTIONS FOR DISCUSSION 



25.1. How would you deline the term provirus? How do the terms merozygote and 

 heterogenote differ? How would you define a homo°enote? 



25.2. What characteristics are conferred upon a host cell infected by a nontransducing 

 temperate phage which becomes a prophage? Does not become a prophage? 



25.3. How would \ou prove that only one exogenote exists in a microcolony of 

 Salmonella produced by an abortive transduction? 



25.4. Discuss the statement: "Temperate phage has chromosomal memory, and the 

 chromosome has temperate phage memory." 



25.5. F particles are known which carry the prophage of A as "memory." How could 

 you prove the existence of such a particle? 



25.6. Describe the procedure and genotypes you would use in demonstrating that 

 E. coli can undergo genetic transformation with respect to Gal. 



25.7. Is there any reason to believe that the close linkage of genes with related effects 

 might be more advantageous in microorganisms than in higher organisms? 

 Explain. 



25.8. List the different ways that the Gal locus in the E. coli chromosome can undergo 

 recombination. 



25.9. Are temperate phages good or bad for bacteria? Explain. 



25.10. Is a cell which has presumably stopped undergoing mutation, genetic recom- 

 bination, and self-replication of its DNA still considered to contain genetic 

 material? Explain. 



25.11. Discuss the origin and relative numbers oi Adg present among the phages in 

 l.FT and HFT lysates. 



