420 CHAPTER 32 



tide-one gone." Thus, we consider thai the amino acid content o\ each polypeptide is 

 specified completely In the primary action of a single gene. The one gene-one pri- 

 mary effect hypothesis is therefore supported, and it is concluded that one way tor a 

 gene to act in a primal \ \\a\ is to specify polypeptide amino acid content. Conse- 

 quently, the \\a\ is opened for the study of evolution at the biochemical level. The 

 molecular evolution of hemoglobin is discussed. 



REFERENCES 



Anfinsen, C. B., The Molecular Basis of Evolution, New York: J. Wiley & Sons, 1959. 



Baglioni, C, "Correlations between Genetics and Chemistry of Human Hemoglobins," 

 Chap. 9. pp. 405-475, in Molecular Genetics, Part I, Taylor, J. H. (Ed.), New 

 York: Academic Press, 1963. 



Beadle, G. W., and Tatum, E. L., "Genetic Control of Biochemical Reactions in Neuro- 

 spora," Proc. Nat. Acad. Sci., U.S., 27:499-506, 1941. Reprinted in Classic 

 Papers in Genetics, Peters, J. A. (Ed.), Englewood Cliffs, N.J.: Prentice-Hall, 

 1959, pp. 166-173. 



Beam, A. G., "The Chemistry of Hereditary Disease," Scient. Amer., 195:126-136, 

 1956. 



Bonner, D. M.. and Mills, S. E., Heredity, 2nd Ed., Englewood Cliffs., N.J.: Prentice- 

 Hall, Inc., 1964. 



Guidotti, G., Konigsberg, W., and Craig, L. C, "On the Dissociation of Normal Adult 

 Hemoglobin," Proc. Nat. Acad. Sci., U.S., 50:774-782, 1963. 



Harris, H., Human Biochemical Genetics, Cambridge: Cambridge University Press, 

 1959. 



Hsia, D. Y.-Y., Inborn Errors of Metabolism, Chicago: Year Book Publishers, 1959. 



Human Genetics, Cold Spring Harb. Sympos. Quant. Biol.. 29, 1965. 



Ingram, V. M., "Gene Evolution and the Haemoglobins," Nature, 189:704-708, 1961. 

 Reprinted in Papers on Human Genetics, Boyer, S. H., IV (Ed.), Englewood 

 Cliffs, N.J.: Prentice-Hall, 1963, pp. 164-175. 



Itano. H. A., and Robinson, E. A., "Genetic Control of a- and /i-Chains of Hemo- 

 globin," Proc. Nat. Acad. Sci., U.S., 46:1492-1501, 1960. 



Kendrew, J. C, "Myoglobin and the Structure of Proteins," Nobel Prize Talk, Science, 

 193:1259-1266, 1963. 



Nance, W. E., "Genetic Control of Hemoglobin Syntheses," Science, 141:123-130, 

 1963. 



Wagner, R. P., and Mitchell, H. K.., Genetics and Metabolism, 2nd Ed., New York: 

 J. Wiley & Sons, 1964. 



Yanofsky, C, and Crawford, L. P., "The Effects of Deletions, Point Mutations, Re- 

 versions and Suppressor Mutations on the Two Components of the Tryptophan 

 Synthetase of Escherichia Coli," Proc. Nat. Acad. Sci.. U.S., 45:1016-1026, 1959. 

 Reprinted in Papers on Bacterial Genetics, Adelberg, E. A. (Ed.), Boston: Little, 

 Brown. 1960, pp. 384-394. 



See Supplement VI and the first portion of Supplement VII. 



