286 EXPEEIMENT STATION EECOED. 



but in no instance was the regnlar acid-fast tubercle bacillus detected. Most 

 of the experimental animals had a generalized tuberculosis. Tlie extracts of 

 casefied foci contained a specific substance which was antagonistic to the 

 tubercle bacillus, and which according to the author does not originate from 

 the bacillus. The use of -iodin preparations in tuberculosis increased the 

 process of congestion. 



Tuberculosis (Illinois Sta. Circ. 158, pp. 3-21, figs. 13). — This is a repub- 

 lication of the compilation previously noted (E. S. R.. 26, p. 378). 



In regard to the chemical composition of the tubercle bacillus, T. Panzer 

 (Hopi)e-8eijler's Ztschr. Physiol. Chem., 78 {1912), No. 5, pp. 4i4-^i9).— The 

 author points out that the published analyses of the tubercle bacillus do not 

 give any clue in regard to the principal constituents of this organism. 



Tubercle bacilli which were cultivated on glycerin bouillon were killed by 

 heat, collected on a filter, washed with water, and dried in a vacuum over 

 calcium chlorid. The weight of dried bacilli obtained was 2.7 gm. They were 

 then rubbed up and extracted successively with ether, alcohol, cold water, 

 1/20-normal hydrochloric acid, 1/20-normal sodium carbonate, 1/20-normal 

 sodium hydrate, hot water, and 2 per cent potassium hydrate. 



The cold water, hydrochloric acid, sodium carbonate, sodium hydrate, and 

 potassium hydrate extracted very little material. The unsaponifiable material 

 contained in the ether extract of the bacilli, precipitated with digitonin and 

 extracted with petroleum ether and xylol, yielded a mixture of colorless crystals 

 and a yellow, smeary mass which did not give the cholesterol reaction. Accord- 

 ing to the author two hi^gher alcohols are probably present. The alcoholic 

 solution obtained re<^luced Fehling's solution and on evaporation yielded a 

 brown, partly crystalline residue. 



The hot water extract of th-e bacilli on evaporation left a residue which re- 

 minded one of gum arable. This substance was free from sulphur, nitrogen, 

 and phosphorus, gave Molisch's reaction with a-naphthol, was precipitated from 

 the aqueous solution with alcohol or lead acetate, and after boiling for a time 

 with hydrochloric acid reduced Fehling's solution. On oxidizing with nitric 

 acid no nuicic acid was produced, the chief product being oxalic acid. In addi- 

 tion a crystalline substance resembling calcium saccharate was also obtained. 

 This substance is probably pectin. 



The residue remaining after extraction of the above reagents and containing 

 nitrogen gave Molisch's reaction, and after boiling with hydrocloric acid reduced 

 Fehling's solution. The filtrate obtained from treating another portion of the 

 residue with pepsin did not give a biuret reaction, although the solid particles 

 remaining did. This resistant body is evidently chltin. 



Tuberculosis of the abomasus of bovines, P. Chauss6 {Bill. Soc. Cent. 

 MM. V6t., 88 {1911), No. 20, pp. ji52-.'t76, figs. 12).— A discussion in regard to 

 the macroscopical and miscroscopical findings and the pathology of this con- 

 dition. The article contains profuse illustrations of pathological preparations. 



The ophthalmic test for bovine tuberculosis, A. Wilson {Jour. Compar. 

 Path, and Ther., 2/, {1911), Nos. 1, pp. 59-66; 2, pp. 116-126).— This is a critical 

 study of the opthalmic and subcutaneous tests for detecting tuberculosis. Con- 

 siderable stress is laid upon the unreliability of diagnosing tuberculosis on the 

 basis of the simple ophthalmic test alone. Satisfactory results can only be ob- 

 tained when both tests are conducted together. 



Utilizing- the work of our international commission on the control of 

 bovine tuberculosis, M. H. Reynolds {Am^r. Yet. Rev., -',1 {1912), No. 2, 

 pp. 196-200). — In this paper the author points out the services rendered by agri- 

 cultural colleges, farmers" institutes, state live-stock sanitary boards, etc., in 

 combating tuberculosis. 



