382 EXPERIMENT STATION RECORD. 



attenuated or virulent bacteria it stimulates a rapid evolution of the pathological 

 lesions in the animals. If the serum is used in large doses it may retard the 

 pathological process, but on the other hand it will have a tendency to favor the 

 elimination of tubercle bacilli through the regular excretory channel. 



The intradermal tuberculin test, D. F. Luckey (Amer. Vet. Rev., Jfl {1912), 

 No. 3. pp. 316-823). — The intradermal test in the hands of this author gave 

 good results, in fact, results somewhat better than those obtained by the old 

 subcutaneous method. The method of making the test is described. The 

 author also points out the value of keeping a history of the animals in the 

 vicinity. 



Diagnosis of pulmonary tuberculosis with regard to the new sanitary laws, 

 O. MtJLLEE {Berlin. Tierdrzil. Wchnschr., 2S {1912). J^'o. 17, pp. 293-295, figs. 

 ''). — This is a description of the Konigsberg method devised by Miiller. Jonske, 

 and Wiemann for diagnosing tuberculosis. This is a modification of the 

 method of Scharr and Opalka (E. S. R., 26, p. 679), but is supposed to possess 

 certain advantages which are described in detail. The author recommends 

 giving an injection of cocaine at the point where the cannula is to be inserted 

 15 minutes before the operation. Reasons for not using the Rautmann and 

 the Tallgren and Graae apparatus are given. 



Sputum gathering methods for diagnosing tuberculosis in bovines, H. 

 Rautmann {Berlin. Tierar.ztl. WcJtnsehr., 28 {1912), yo. 19, pp. 335-337).— 

 This is a polemic in regard to Miiller's method (see above) for obtaining 

 sputum. The author also discusses the value of his own apparatus, which is 

 a modification of the Tallgren and Graae apparatus (E. S. R., 24, p. 84). 



Preliminary report upon the injection of rabbits with protein-free (tuber- 

 culo) antigen and antigen-serum mixture, A. H. Caulfeild {Proc. Roy. Sac. 

 [London], Ser. B, 8J, {1911), No. B 573, pp. 390-39/,).— This work is sum- 

 marized as follows : 



"The injection of rabbits with a saline dilution of an alcohol-ether extract 

 of tubercle bacilli is capable of producing complement-fixation bodies to that 

 antigen. The digestion at 37.5° C. of the alcohol-ether extract with inhibitive 

 serum (human) for various intervals up to 1 hour does not affect the pro- 

 duction of the specific sensitizers. The digestion at 37.5° of the alcohol-ether 

 extract with fixation-positive serum (rabbit) for 1 hour inhibits the produc- 

 tion of specific sensitizers. . . . 



" The injection of ' bacillen-emulsion ' in 2, 1.5, 0.5, and 0.005 mg. amounts 

 failed to induce fixation bodies. The injection of 100 mg. of partially dried 

 dead tubercle bacilli (after alcohol-ether extraction) produced after the first 

 injection sensitizers, so that 0.1 cc. serum caused complete fixation although 

 the injection of 0.5 mg. failed to do this, . . . 



" The rabbit's blood was obtained by puncture of the ear vein and withdrawal 

 by a 2 cc. record syringe. This has been found to be the most rapid and satis- 

 factory method of obtaining up to 5 cc of blood. The needle and syringe are 

 sterilized in hot oil, the needle being then well cleared in cold sterile saline." 



The results obtained in the eradication of tuberculosis from a herd by the 

 use of tuberculosis vaccine and the Bang system, S. H. Gilliland {Ann. Rpt. 

 Penn. Dept. Agr., 16 {1910), pp. 166-179; Proc. Amer. Vet. Med. Assoc, 48 

 {1911), pp. It77-Jf92). — This is the report of a series of experiments made for 

 the purpose of eradicating tuberculosis from a herd, which originally consisted 

 of 200 animals, comprising Holsteins, Guernseys, and gi'ades, on the basis of 

 the tuberculin test and the Bang method. 



In 1904, when the tests were begun, 160 animals, which constituted the milk- 

 ing herd, were tested and showed 42 reactors, or 26.2 per cent. Nearly all of 

 the reacting animals were in various periods of gestation and kept in separate 



