48^ EXPEEIMENT STATION RECORD. 



Combating bovine tuberculosis according to the new epizootic laws in 

 Germany, E. Schaee (Berlin. Tierdrstl. Wchmchr., 28 (1912), No. 20, pp. 3/^9- 

 353). — A discussion of the methods for diagnosing tuberculosis in bovines in 

 Germany. Various phases of the law are critically discussed, including the re- 

 imburshig of the owner of tuberculous cattle. 



Experience in eradicating tuberculosis from a herd, N. S. Mayo {Bpt. V. S. 

 Live Stock Sanit. Assoc., 15 {1911), pp. 186-191). — This is a brief history of an 

 outbreak of tuberculosis in the herd of the Virginia Polytechnic Institute, to- 

 gether with advice in regard to eradicating tuberculosis from herds. 



Cultivation of the bacillus of chronic intestinal inflammation of the 

 bovine (paratubercle bacillus), H. Holth (Ztschr. Infektionskrank. u. Hyg. 

 Haustiere, 11 (1912), No. 5, pp. 378-387, figs. 6; ahs. in Jour. Compar. Path, 

 and Ther., 25 (1912), No. 2, pp. 148-150). — In the author's investigation of 

 Johne's disease cultures obtained from a case which reacted to avian tuberculin 

 were planted upon 7 types of media. The most abundant growth was ob- 

 tained on media consisting of blood serum with i volume of liver broth, 2 per 

 cent dead tubercle bacilli, aud 4 per cent glycerin. 



"At 6 weeks the whole surface of the medium was covered with colonies that 

 were visible to the naked eye, and which later attained a size varying from * 

 to 1 mm. By reflected light the colonies were greyish-white in color, and by 

 transmitted light yellowish-brown. They were circular in outline, and under 

 a low magnification the surface was observed to present a slightly wrinkled 

 appearance. The colonies were tough. In older cultures there was observed 

 the formation of a thin delicate membrane of wrinkled growth extending round 

 the colonies. Growth on the other media presented similar appearances, but 

 developed more slowly. Subcultures on the same medium yielded quite abun- 

 dant growth in about 4 weeks." 



The inoculation of guinea pigs failed to induce the disease, producing at most 

 a small encapsuled abscess at the seat of inoculation, the contents of which 

 contained a few granular acid-fast bacilli. Experiments with rabbits gave 

 similar results. In experiments with tuberculin-tested calves, inoculations made 

 subcutaneously and intravenously caused no reaction. Three months later the 

 calves were tested with ordinary tuberculin and did not react but with avian 

 tuberculin typical reactions were obtained at the end of 3 weeks. 



" Experiments were carried out with guinea pigs with the object of ascertain- 

 ing whether Johne's bacillus is capable of conferring any immunity against 

 tuberculosis. The animals received 2 comparatively large doses of culture, and 

 a month later a dose of bacilli of the bovine type. Control animals were in- 

 oculated with the bovine bacilli alone. All the animals were weighed twice 

 weekly, and it was found that the control animals commenced to lose weight 

 sooner than the others. The author concludes that a slight degree of immunity 

 was established." 



The anaplasmoses of cattle, L. E. W. Bevan (Vet. Jour., 68 (1912), No. 445 

 pp. 392-400). — This is a general discussion of the subject. It is pointed out 

 that while trypanblue is invaluable in checking piroplasmosis it is ineffective 

 against anaplasmosis, which in the present state of our knowledge can be com- 

 bated only by careful nursing and a diet which can be digested, assimilated, 

 and made use of to replace and repair the loss of tissue caused by the disease. 



Anaplasmosis of sheep, L. E. W. Bevan (Vet. Jour., 68 (1912), No. 445, pp. 

 400, 401)- — The author reports the occurrence of this disease among sheep. He 

 states that it has a wide distribution throughout Rhodesia. 



In regard to a vaccine prepared by a new method against hog cholera and 

 swine plague, Krafft (lici'Un. Ticrarztl. ^]'cln^schr., 28 (1912), No. 15, pp. 

 261-266). — The preparation of the vaccine is based on the following principle: 



