578 EXPEEIMENT STATION KECOED. 



It was found that the catalytic power was mcreased at the beginning of the 

 disease and later returned to normal, so that this is to a certain measure an 

 indication of the gi'avity of the condition. The reducing power (reductase) 

 was determined by the authors' method, i. e., an aqueous solution of methylene 

 blue 1 : 40, and an aqueous alcoholic solution of fuchsiu 1 : 4,000. In most of the 

 cases the reducing time was 3 hours or over at the outset and did not vai-y 

 very much during the course of the disease, but in some instances the reduction 

 time was lessened at the outset, though afterward increasing, and taking as a 

 rule more than 3 hours. 



The significance of the agglutination-complement fixation and the con- 

 junctival test for the diagnosis of glanders, H. Miessneb (CentM. Balct. 

 [e/0.], 1. Am., Orig., 03 (1912), No. 7,-6, 2W- 482-5.'i2, figs. 6).— The height of 

 the agglutination titer and complement-fixing values of the serum of glandered 

 animals stands in direct relation to the age of the pathologic process but not to 

 its extent. The author does not consider fixing values of 0.3 and 0.4 for the 

 serum of horses in stables free from glanders to be suspicious, but does when 

 found for the serum from animals in infected barns. It is also advisable to 

 make the second or third tests 10 days apart. 



The conjunctival test was used with 133 horses, among which were 59 clini- 

 cally affected with glanders. Of these 59. 53 showed a positive ophthalmic test 

 while none of the 74 horses classed as sound reacted. With the conjunctiA'al 

 test a reaction can be completed within from 14 to 20 hours after instilling the 

 malleiu, and when in doubt as to the results the test can be repeated within 24 

 Lours and with the same eye. 



A comparative study with the agglutination, conjunctival, and complement 

 fixation tests was made with 74 horses for the purpose of determining the value 

 of the tests. With the first named test 88.8 per cent of the horses which were 

 positively glandered were detected ; M'ith the complement fixation method 100 

 per cent ; and with the conjunctival test 94.4 per cent. With the last two 

 methods no sound horses were diagnosed as being glandered. 



A previous conjunctival-mallein test has no influence upon a subsequent 

 agglutination test, although it seems to increase slightly the complement fixation 

 values. The agglutination, complement fixation, and ophthalmic tests were 

 studied with horses artificially infected per os and subcutaneously. The con- 

 junctival reaction was noted from 5 to 9 days after infection, the agglutination 

 test in from 5 to 7 days, and the complement fixation test in from 10 to 14 days 

 after infection. Agglutination or complement-fixing bodies are not transmitted 

 by the mother to the fetus. 



Critical investigation in regard to the diagnostic value of the ophthalmic 

 reaction in glanders, E. Frohneb (Monatsh. Prakt. Tierheillc, 28 {1911), No. 

 1, pp. 1-35; abs. in llyg. Rundschau, 22 (1912), No. 11, p. 7Zi).— The ophthalmic 

 reaction was found very valuable for diagnosing glanders in horses. It was 

 tested with 21 animals, which were undoubtedly glanderous, at the internal 

 clinic of the veterinary high school at Berlin. The method was conducted by 

 histilling from 1 to 2 drops of a mallein solution (0.05 gm. of mallein siccum 

 [Foth] in 4.5 cc. of a 0.5 per cent solution of carbolic acid). After 12 hours a 

 pronounced, thick purulent secretion was produced, which is not obtained with 

 sound horses. 



Experimental therapy of Rocky Mountain spotted fever, P. G. Heinemann 

 and J. J. Moore (Jour. Inject. Diseases, 10 (1912), No. S, pp. 29J,-30J,).— 

 " Horses are susceptible to spotted fever if the virus of guinea pigs is injected 

 f^ubcutaneously and intravenously. The fever usually talies a mild course and 

 the temperature is not exceedingly high. Normal temperature appears agaiu 



