4fi2 



fultnres the sports werp pla««'d iip*>ii pieci-s ofnlate rut to fit into the tiinihlfmaltont 

 an inch l»«'lo\v tht- tup, and jjirforati-d to allow th<* roots to run t)irou<;h to th«- watft 

 below. Six of these water <iiltures were in water alone, an<l six iu water to wliirh 

 a ta1ilesj)ooiiful of conimoM mnipost was a<lded as a fertilizer. In each m-t two wer« 

 of distille<l water, two of rain water, and two of hard water. 



The same general plan was fcdiowed with the pots of san«l and soil, respeetivelv, 

 exc-ept that alternate jiots were snhject to jirolunj^ed heatin;; in order to destroy all 

 jjernis whieh they may havi' eontained. • • • Kxaniinations <if the routs for 

 tuhereles were made May 13, when the plants were 'A to 4 inehes hi;;h and well estab- 

 lished; May 2><, just lieture blossoming;; .June l.'>, when. just be^innin;; to fruit; and 

 July li, when full <;rowth was att.iined anil they were be;;inninK '" decline. 



Tlic icsiiltsarc t;iliiil;it<il ;iinl <lis(ii.ss«Ml, There an* alsit l»ri«'rn*iH)rts 

 oil niltmes nia«le in the plant lnni.>*e in NnveiiiJuT ami .laniiary. The 

 exjieriiiieiits tliiis far made are .^iiniiiiari/ed as foIh>\vs: 



(1) Rout tubereles did not ap]ie:ir iu water cultures uf any kind. 



(2) While the cultures in a sterilized nu-diuin. supplied only with distilled watrr. 

 were not entirely devoid id" tubercles, they \\eri' produced more tardily, were less 

 abundant, and a less number of the cultures cuntaiucd them. 



(3) On the other hand the addition of a fertili/.er rendered the )>roduction of tuber- 

 cles more ra|>id, nuire abundant, and in a ;:rc:iter number of culture.n. 



(4) As to relativi! abundance of tubercles in sand or soil, the results were cunllict- 

 injj. In K''"<'r"l the better develujied the plant the better develojMMl the tubercles. 



(ri) The inei|uality uf the distribntiun uf tubercles u]ton the roots was very strik- 

 inj;. In some eases they were cnntiiied to a sinjjle one of a scure or more of roots :ill 

 ei|ually well ])laced. Krei|Ueutly all which a jdant bore were n|MUi t\M> i>r three 

 roots only, all the utlurs beiu;; entirely free frum them. 



Where routs ran thruu^h the pots into the cinders upon which tin- puis were 

 ])laceil, tubercles were in several places >;reatly develuped. beinj; larj^iT and mure 

 iiumeruus than i>u the ruuts within. 



,\ careful examiuat iun uf I he ruuts uf two species of I'ti^olftiiun and uue uf MimnKrtr 

 in addition to Ihusc mentiuned in the ]ireviuus repurt, jjave nu tubercles whjit<'V<T, 

 while nil ca.se uf their absi-nce frum the ruids of I'njiilinuitno lla^ \ct come under my 

 (di.servaliuu. 



Tin: PKKIODKAL rir.ADA IN I'KNNSVI.VAM A. W . .\. HirKHolT. .M. 

 S. (|»i). 1SL'-1S7). — All accuiiiit of the life liistiirv anil lialiits uf Cirtifln 

 xrptriinlrrim. with .i in;i|» -^liitw iii;,^ tin- ilistrilmtiiniof hriMuls in IN'iiii.syl- 

 vania as ijetrnniiicti l>.\ ri'i»Iirs to a eirnilar of iiii|iiiry snit out from 

 the station. 



Invksih;ati(»ns i imin ^ikthods iok disi uiminatim; hktwkk.n 



PHosrilAIKS DKK'IVKI) I'b'nM HnNF, WD lllnsr, DKKIVKD FROM 



Soirii ("Ab'oi.iNA imcK. W. I-'ri:ai;. I'm. D. (pp. I.S.S-i;Mh. — lirief 

 nifiition is mailr of thr in\esii;Xat ions o| von I/omi/.* ami Stockla.«<at 

 on the valiir for tliis piuposr of thr ih-trnninat ion of tii<> tliiorine in 

 ]>hosplial it- fntiii/ris, ainl thi- lonlts air ;;i\riMd' ilrtriininalions of 

 the iron ami silira in six samples eaeh ot' ilissolvctl roi-k ami hone. 



The hijihest aiuuuul <d' iron found in any of the bone fjuods w;is (l.liT per cent, 

 while in the dissohfd ruck the minitnntn waa 1.4 per rent. It would certainly seem, 



* Oesterr-l'u;;ar. Zeitsch. f. Kiiben/ucker-Iud., 17, p. "JTO; abs, in t'entralbl. f. agr. 

 Cheni., IS. p. i:iO. 



tC'flitralbl.f. agr. t'hcni., is,, p. 111. 



