80 EXPERIMENT STATION RECORD. [Vol. 3« 



procedure (E. S. R., 26, p. 22) yielded the following figures: Ammonia, 7.61; 

 melanin, 4.95; arginin, 5.81; cystin, 1.17; histidln, 8.29; lysin, 3.46; amino 

 (in filtrate from bases), 37.3; and nonamino (in filtrate from bases), 11.17. 

 It is indicated that the results do not show anything unfavorable in regard to 

 the hydrolytic products of the soy-bean hay. 



A substance soluble in 70 to 80 per cent alcohol and water was obtained from 

 an alcoholic extract of the hay by precipitation with basic lead acetate. It was 

 toxic for guinea pigs when fed with the food or injected subcutaneously. An 

 extract obtained from alfalfa hay by a similar procedure had no effect when 

 injected subcutaneously into a medium-sized guinea pig, thus indicating the 

 material to be specific for soy-bean hay. The material obtained from the ex- 

 tract contained 19.35 per cent dry matter and 0.3 per cent nitrogen. A deter- 

 mination of the nitrogen distribution of the material was made, but was only 

 partially successful. 



Studies on antibodies. — I, Analyses and nitrogen distribution of a number 

 of antisera, B. J. Banzhaf, K. SucnjKA, and K. G. Falk (Collected Studies Bur. 

 Lab. Dept. Health N. Y. City, 8 (1914-15), pp. 2i5-222).— Analytical data on 

 the hydrogen-ion concentration, moisture, ash, phosphorus pentoxid, and nitro- 

 gen of the following antisera are submitted in tabular form, together with the 

 formol titrations : Anti-diphtheria globulin, anti-tetanus globulin, anti-tetanus 

 serum, anti-streptococcus serum, anti-gonococcus serum, anti-meningococcus 

 serum, and normal serum. 



Data obtained from the analysis of normal serum, tetanus globulin, diph- 

 theria globulin. Hartley's whole protein, euglobulln (ammonium sulphate 

 method), and euglobulin (Panum's method) by the Van Slyke procedure (E. S. 

 R., 26. p. 22) are also reported. 



No very marked differences in the composition of the different preparations 

 were found. The only points to which attention is called are the higher values 

 for cystin of the tetanus and diphtheria globulins. 



The intrapalpebral mallein test, Goodall (Jour. Compar. Path, and Ther., 

 28 (1915), No. 4, pp. 281-297, figs. >}).— The results of the tests of a large num- 

 ber of military horses in Southwest Africa, together with the post-mortem 

 findings of a number of the animals, are reported. 



The Intrapalpebral test is deemed a safe and reliable method in the diagnosis 

 of glanders and far superior to the ordinary subcutaneous procedure. The 

 advantages claimed for it over the classical subcutaneous method are sum- 

 marized as follows: 



Clinical cases give constant reactions to this method. Animals which had 

 given double reactions to the subcutaneous test reacted to this method even 

 during the height of the subcutaneous temperature curve. Doubtful reactors 

 to the subcutaneous test can be retested by this method, and give reactions 

 immediately afterward. Certain animals which have failed to react to the 

 subcutaneous test, or which could not be tested by it on account of other dis- 

 eases and high temperatures, can be tested by this method, and react if glan- 

 dered. A safe diagnosis can be made on the local reaction alone. The local 

 reaction is more delicate, and doubtful reactions are extremely rare. In ordi- 

 nary cases temperature, local, and ophthalmic reactions can be obtained by 

 one injection. Mules react to this test as readily as horses. 



The reliability of cell proliferative changes in the diagnosis of rabies, 

 J. B. Hardenbergh and B. M. Underhill (Jour. Amer. Vet. Med. Assoc., ^9 

 (1916), No. 5, pp. 66S-668, pis. S). — Investigations made of a large number of 

 cases of suspected rabies have led to the conclusion that Negri-like bodies 

 may occur in smear and section preparations from brains of animals dead 

 from other causes than rabies and also in association with Negri bodies in 



