2Y6 EXPERIMENT STATION RECOBD. [Vol.36 



306-314, figs. 6). — A new appai'atus and its use and manipulation in the isola- 

 tion of living organisms are described in detail. 



Lupines as poisonous plants, C. D. Maesh, A. B. Clawson, and H. Maesh 

 (U. S. Dept. Agr. Bui. ^05 {1916), pp. U, Vls. If, figs. 2).— The introductory part 

 of this bulletin gives a summary of knovpledge of lupines as poisonous plants 

 and accounts of their distribution, common names, alkaloids, etc. The second 

 part (pp. 7-28), which deals with experimental work, takes up pharmacological 

 investigations by Sollmann, field experiments with lupines, and laboratory ex- 

 periments with extracts of lupine seed upon mice. The third part (pp. 28^2) is 

 devoted to a discussion and general conclusions. 



"An investigation by Sollmann showed the presence of alkaloids in American 

 lupines, and pointed to the probability that most, if not all, the poisoning of live 

 stock in America was due to these alkaloids and not to ictrogen. Extended 

 field work has verified the conclusions of Sollmann and has shown that all aerial 

 parts of the lupines examined are poisonous, the seeds being the most toxic, then 

 in order the pods- and leaves. This has been- confirmed by preliminary experi- 

 ments with extracts upon- mice. 



" The toxic substance is excreted* by the kidneys ; the intoxication is not 

 cumulative, and animals may eat comparatively large quantities with no ill 

 results, if the toxic limit is not reached at any one time. Inasmuch as the toxic 

 and lethal limits are nearly the same, the prognosis for poisoned animals is not 

 favorable. There is no form of remedial treatment that can be used advan- 

 tageously for range animals. Poisoning in most cases can be avoided, even 

 where the plant is abundant, by careful handling of the flocks, especial care 

 being taken to see that hungry siieep are not grazed on fields where there is 

 much lupine." 



A list of 29 references to the literature is included. 



Studies on tha action of glycerin. — I, The hemolytic action of glycerin in 

 vitro and in vivo, I. Simon {Arch. Farmacol. Sper. e Sci. Aff., 20 {1915), Nos. 

 S, pp. 120-l.',Ji^ figs. 4; 4, pp. 145-165, fig. 1; abs. in Chem. Zentbl., 1916, J, 

 No. 1, p. 25).^The blood of cattle and rabbits was hemolyzed by glycerin in 

 all concentrations. The time necessary for hemolysis was found to increase with 

 increasing concentrations of glycerin, and to reach a maximum at a concentra- 

 tion of 55 per cent for cattle blood and 50 per cent for rabbit blood. With in- 

 creasing concentrations of the glycerin the rate of hemolysis increased rapidly. 



Subcutaneous and intraperitoneal injections in rabbits produced serious local 

 lesions and a general toxicity. When the amount of glycerin injected exceeded 

 2.5 gm. per kilogram of body weight a hemoglobinuria resulted, regardless of the 

 strength of the glycerin injected. 



The theory of the action is discussed. 



A corktribution to the action of amino adds, peptids, and proteins on 

 hemolysis by cobra venom, E. Zunz and P. Gyoegy {Jour. Immunol., 1 {1916), 

 ^0. 5, pp. 5Sl-o69). — Detailed experimental results are submitted which show 

 that the effects of amino acids, peptids, and proteoses on cobra venom hemolysis 

 vary according to the species of blood corpuscles used and also the amount in 

 the blood-venom mixture. 



" The hemolytic -action of cobra venom on the red cells of guinea pig's 

 blood is markedly hastened and increased in intensity t)y phenylglycocoU, 

 leucin, glycyltryptophan, and protoalbumose. The hemolysis is produced more 

 quickly in the presence of large quantities than it is with small amounts of 

 phenylglycocoU and leucin. Heteroalbumose tends slightly to increase the in- 

 tensity of the hemolysis ;' alanin, on the contrary, diminishes it a little. Glyco- 

 coll and particularly diglycin, triglycin, and leucylglycin inhibit the hemolysis 

 in direct proportion to their concentration. 



