19171 VETERINARY MEDICINE. 575 



can be satisfactorily packed in bran. For larger samples wrapping in cotton 

 cloth previously soaked in alcohol is recommended. The use of vinegar, mer- 

 curic chlorid, pickling solutions, borax, and bleaching powder is not recom- 

 mended as practical, although in isolated cases the experimental results obtained 

 were satisfactory. 



Serums, vaccines, and toxins in treatment and diagnosis, W. C. Bosanqxiet 

 and J. W. H. Eybe {Ne%v York: Funk d Wagnalls Co., 1916, 3. ed., pp. VIII+456, 

 figs. 20). — This volume discusses the subject under the general topics of im- 

 munity and resistance to disease, preparation and administration of sera and 

 bacterial vaccines, sera and toxins in diagnosis, diphtheria, tetanus, snake bite, 

 hydrophobia, smallpox and vaccinia, anthrax and glanders, plague, cholera, 

 enteric fever, dysentery and other bacillary infections, tuberculosis, leprosy, 

 affections due to streptococci, other infections due to cocci, catarrhal affections, 

 diseases due to protozoa, and m.alignant tumors. An appendix on various 

 conditions treated with sera is included. 



Digested and diluted serum as a substitute for broth for bacteriological 

 purposes, A. Distaso {Brit. Med. Jour., No. 2912 (1916), pp. 555, 556).— The 

 author describes the preparation of a culture medium from sheep or ox serum 

 by digestion overnight with an aqueous extract of pig's pancreas previously 

 activated with an aqueous extract of a small portion of the duodenum of the pig. 



A luxuriant growth of the Bacillus coli group, streptococcus, staphylococcus, 

 B. subtilis, B. proteus, and B. fluorescens was obtained on the medium. Little 

 or no growth of meningococci was obtained, however. 



For indol foi'mation the medium yielded the same results as the usual trypto- 

 phane medium. The medium is also suited for sugar tests and by combining 

 with agar can be used to produce a beautiful transparent solid medium. 



Directions for the preparation of the diluted serum for sugar tests are also 

 submitted. This medium is especially suitable for B. coli and is much cheaper 

 than those commonly used. 



The effects of serum treated with agar, E. Zunz and M. Gelat {.Jour. Expt. 

 Med., 24 (1916), No. 3, pp. 247-269). — Experiments reported in detail show that 

 the intravenous injection into normal rabbits of horse serum, previously kept 

 for two hours at 38° C. in the presence of 0.2 of its volume of a 0.5 per cent 

 suspension of agar in physiological saline and then separated from the agar by 

 oentrifugalization and filtration, produces a considerable and prolonged fall in 

 blood pressure, expulsion of feces, diminished coagulability in -the carotid blood, 

 and, at times, accelerated respiration. The various symptoms produced are the 

 same as those observed after the intravenous injection of horse serum into a 

 previously sensitized rabbit. 



Horse serum previously kept for 30 minutes at 56°, and then treated with agar 

 as described, produces no other effect than does the ordinary introduction of 

 horse serum into the veins of a normal rabbit. 



The experimental results are discussed in some detail. 



The effect of moderately high atmospheric temperatures upon the forma- 

 tion, of agglutinins, C. E. A. Winslow, J. A. Miller, and W. C. Noble (Proc. 

 Soc. Expt. Biol, and Med., 13 {1916), No. 8, pp. 194-197). — The results of experi- 

 ments reported, in which animals were immunized by successive intraperitoneal 

 injections of increasing doses of killed typhoid bacilli and kept at temperatures 

 ranging from 29 to 32° C, indicate that the moderately high temperatures 

 tend to lower the power of agglutinin formation in rabbits. 



Immunity produced by intravascular injections, L. Camxts {Compt. Rend. 

 Acad. Set. [Paris], 163 {1916), No. 14, pp. 338-340). — Experiments are reported 

 which show that the time necessary for immunization varies with the dose of 

 vaccine employed. It is not possible, as some have supposed, to suppress the 



