484 EXPEETMENT STATION RECORD. [Vol. 38 



abs. in Trop. Vet. BuL, 5 {1011), No. 3, p. 155). — "Nineteen dourine sera gave 

 positive results corresponding with those given by the ordinary deviation of 

 the complement test, and to a large extent with the agglutination test, with 

 one exception, in which an animal that had been infected for a considerable 

 time and had then been treated with atoxyl ceased to give a positive result with 

 the complement test, but continued to react strongly according to the congluti- 

 nation test. Thirty normal sera gave negative results, except at first in two 

 cases where errors of technique were detected." 



The author's conclusion is that the conglutination test is applicable for the 

 diagnosis of dourine sera, but that it is more sensitive to errors and therefore 

 more difficult to carry out than the ordinary deviation of complement method. 



Studies on rinderpest, H. Schein (Ann. Inst. Pasteur., 31 (1911), No. 11, 

 pp. 511-592).— Thin article reports a series of studies on the vaccination of 

 cattle and buffaloes against rinderpest. The work was undertaken because of 

 the unfavorable results with buffaloes of the usual vaccination by simultaneous 

 injections of serum and virus. In the investigations recorded, horses were 

 used as the experimental animals. 



The earlier conclusions of Kolle and others that the virus is located in the 

 blood corpuscles were confirmed. The virus was found, for the most part, 

 in the leucocytes but to a slight extent in the plasma. Dilution experiments 

 proved that 0.001 cc. of virulent blood constituted a fatal dose and 0.00004 cc. 

 the limiting infecting dose. Virus was present in 0.1 cc. of centrifuged citrated 

 plasma. 



Experiments with specific and nonspecific serums showed that (1) the non- 

 specific serum of the horse has no action on the rinderpest virus, (2) the 

 serum of certain normal cattle can attenuate or kill the rinderpest virus of the 

 horse, (3) the antirinderpest serum probably acts upon the organism of the 

 animal injected and not upon the microbe itself, and (4) the success of vacci- 

 nation depends on the proportion of serum to virus. 



The following method of vaccination of cattle and buffaloes during an 

 epidemic of rinderpest was successfully used: The cattle received, according to 

 size and age, from 40 to 80 cc. of serum ; the buffaloes from 100 to 160 cc. 

 Both cattle and buffaloes received 1 cc. of a dilution of virxilent blood of 1 to 

 1,000. The diseased animal (the source of the blood) was punctured in the 

 jugular vein with a small 2 cc. syringe and 1 cc. of blood withdrawn and placed 

 in 1 liter of water containing 8 gm. of sodium chlorid and 2 gm. of sodium 

 oxalate. The solution was boiled and cooled and was used within half a day. 

 It was probable that all the animals inoculated liad been infected, but very 

 few deaths occurred in the six villages where vaccination was employed, and 

 the epidemic was completely arrested. 



The author concludes from his experience that serum infection appears to 

 give the best results in buffaloes by injecting a sufiicient quantity of serum 

 (about 50 cc. per 100 kg. for adults and double the amount for young animals), 

 and infecting it with the least possible amount of virus to retard the growth 

 of the parasite. 



Rocky Mountain spotted fever in California, J. G. Gumming (Jour. Infect. 

 Diseases, 21 (1911), No. 5, pp. 509-514, figs. 5). — The results obtained from the 

 inoculation of animals has definitely established the occurrence of Rocky Moun- 

 tain spotted fever in California, while the finding of tlie tick Dermacentor 

 venustus in Ventura County and the occurrence of a ca.se there marks that 

 region as a new area of possible prevalence of the disease in the State. 



Experimental trypanosomiasis: T. equiperdum infection in the dog', E. B. 

 Kbumbhaab {Jour. Infect. Diseases, 22 {1918), No. 1, pp. 3^-42, figs. 5). — " Dogs 



