1918.] VETERINARY MEDICINE. 583 



by the addition of cholesterol or sodium oleate the appearance of the agglom- 

 erations can be hastened or retarded, and simultaneously the disappearance of 

 the toxicity of the serum hastened or retarded. 



Experiments in the differentiation of blood and muscular albumin by 

 precipitation and anaphylaxis, C. Lopex (Amer. Jour. Yet. Med., 12 (1917), 

 No. 12, pp. 853-857). — The processes at present employed for obtaining serums 

 for the differentiation of the albumin of blood serums are reviewed, and the 

 following conclusions are drawn from the author's investigations of possible 

 methods : 



(1) To obtain precipitating serums for blood albumin, the best method is 

 that of intravenous injections of 20 cc. each of horse serum into rabbits weigh- 

 ing at least 3 kg. (2) For the differentiation of albumin of the muscles, the 

 best and most easily obtained and preserved antigen is that obtained by adding 

 sodium chlorid to minced meat and dialyzing the juice through parchment 

 paper. 



When a greatly accentuated degree of specificity is required, as in dealing 

 with meats denaturalized by heat, the author has found anaphylaxis reactions 

 superior to precipitins for the differentiation of albumin. 



A new enzym of the leucocytes of blood and of pus, lipoidase, N. Fies- 

 siNGEB and R. Clogne {Compt. Rend. Acad. Sci. [Parisl, 165 {1917), No. 21, pp. 

 730-732; abs. in Chem. Abs., 12 (1918), No. 7, p. 705).— The authors have demon- 

 strated that the leucocytes of the blood and acute suppurations have the power 

 of secreting an enzym (lipoidase) which hydrolyzes lecithin. The enzym is 

 destroyed by heating to from 56 to 60° C. for 30 minutes. It does not act in 

 strongly acid or alkaline solutions but preferably in a slightly alkaline medium. 

 The action is inhibited by formalin, by red corpuscles in large quantities, and 

 prol)ably by normal serum. The enzym has been identified in normal leuco- 

 cytes of the blood of man, the dog, and the cat, and in aseptic and septic ab- 

 scesses. It is present in poly nuclear cells and abseut in the lymphocytes o' cer- 

 tain chronic effusions, as in pleurisy. This lipoidase can be distinguished from 

 the leucocytic lipase by its greater thermostability. 



Studies of the blood fat and lipoids of the dog before and after the pro- 

 duction of experimental anemia, H. Dubin (Jour. Biol. Chem., 33 (1918), No. 

 S, p. 577). — By the use of the nephelometric and colorimetric methods of Bloor 

 (E. S. R., 34, p. 563; 35 pp. 13, 166), blood fats have been estimated in a dog 

 before and after infection with Trypanosoma equiperdum. The results shon' 

 that in trypanosome anemia the total fats are increased while the lecithin and 

 cholesterol are decreased. These results are in agreement with the reports 

 of Bloor (E. S. R., 36, p. 365) in pernicious anemia associated with carcinoma 

 of the stomach. 



Immunity and tissue transplantation. — I, The reactions occurring about 

 tissue transplanted into heterologous animals, M. S. Fleisheb (Jour. Med. 

 Research, 37 (1918), No. 3, pp. 483-497 ) .—The experiments reported were car- 

 ried out in normal and immune rabbits into which pieces of guinea-pig kidney 

 were transplanted. The rabbits were immunized by repeated intraperitoneal 

 injections of sterile emulsions of kidney cells. In both the normal and im- 

 munized rabbits pieces of guinea-pig kidney removed from the living animal 

 were immediately transplanted under aseptic precautions into subcutaneous 

 pockets on the abdomen of the rabbit. The pieces were removed and examined 

 at various intervals up to 28 days. The examination showed the following 

 general differences between the pieces in normal and in immunized animals : 



" Kidney of guinea pigs transplanted into normal rabbits remains alive and 

 shows regeneration even at 28 days. No regeneration takes place in immune 

 animals. Leucocytes collect in larger numbers about the transplanted tissues 



