1918.] VETERINARY MEDICINE. ' 887 



utes in a water bath at 80° C. It is then centrifuged for 20 to 30 minutes, 

 and the clear liquid is ready for injection in doses of from 2.5 to 3 cc, accord- 

 ing to the technique employed for the mallein reaction. 



Pyotherapy in epizootic lymphangitis, A. Lanfranchi and P. Baki)ei.li 

 (Mod. Zooiatro, Parte Sci., 28 (1917), No. 12, pp. 261-275, figs. 3). —The author 

 reviews the literature on the treatment of epizootic lymphangitis by pyo- 

 therapy and reports unsuccessful results of using this method in cases of 

 light, moderately severe, and severe lesions. He concludes that pyotherapy 

 or autopyotherapy does not represent a method for the definite cure of epi- 

 zootic lymphangitis. 



A study of hemorrhag'ic septicemia: Observations in sheep and in moufloii- 

 sheep hybrids, N. Mori (R. 1st. Incoragg. Napoli, Ann. Staz. Sper. Malattie 

 Infctt. Bestiame, 3 (1916), No. 2, pp. 3-35; abs. in Intcrnat. Inst. Agr. [Rovie], 

 Intcrnat. Rev. Sci. and Pract. Agr., 8 (1917), No. 7, pp. 1010-1012;, Abs. Bad., 

 1 (1917), No. 6, p. 529). — ^A short history of the disease as reported by various 

 investigators is given followed by a detailed study of it as observed in mate- 

 rial from the Naples Veterinary School and other districts of southern Italy. 

 The symptoms and anatomical lesions in subacute and chrome forms of the 

 disease are described. 



The organism Bacillus ovisepticus, responsible for the disease, is polymor- 

 phous, Gram-negative, nonmotile, nonspore-forming, and aerobic. Immune 

 serum prepared with it agglutinates the strains of hemorrhagic septicemia of 

 other animal species, thus justifying the interpretation that the various strains 

 are races of a single organism derived through acclimatization in organisms of 

 another species. For the treatment of the disease the author refers to the 

 serum vaccine treatment of Raebiger (E. S. R., 35, p. 77) and others. 



A bibliography of 14 titles is appended. 



The enzyms of the tubercle bacillus, H. J. Corpek and H. C. Sweany (Jour. 

 Bact., S (1918), No. 2, pp. 129-151, figs. S). — Studies on the autolysis of tubercle 

 bacilli, previously noted (E. S. R., 36, p. 181), were continued by the determi- 

 nation of autolysis of bovine tubercle bacilli and a determination of the a-amino 

 nitrogen in the autolysate. It was found that " tubercle bacilli of both the 

 human and bovine varieties possess autolytic enzyms, as indicated by the non- 

 coagulable nitrogen and a-amino acid nitrogen liberated at incubator temijera- 

 ture after the bacilli have been killed by toluene and chloroform." 



The presence of individual enzyms in the tubercle bacillus was determined 

 by the nephelometric method of Kober and Graves (E. S. R., 32, p. 310) and 

 by examining the autolysate for the various products of enzym action. It 

 was found that " the bacilli themselves, or autolysates therefrom, also possess 

 a trypsin-like enzym capable of splitting proteins in alkaline solution, an 

 erepsin-like enzym capable of decomposing peptone in acid solution, a weak 

 pepsin-like enzym capable of splitting proteins in acid solution, a nuclease 

 capable of splitting nucleic acid, and a urease capable of decomposing urefx. 



" The tubercle bacilli, or autolysates therefrom, do not possess enzyms ci;pa- 

 ble of hydrolyzing starch or inverting sucrose, demonstrable by the delicate 

 Lewis and Benedict picramic acid method. Autolysates from tubercle bacilli 

 do not possess enzyms capable of digesting elastic tissue prepared from lamb 

 lung, or connective tissue prepared from tubercles, at least as indicated by the 

 methods used for demonstrating these enzyms." 



Bovine tuberculosis: Its diagnosis and control, V. A. Moore (Amcr. Jour. 



Vet. Med., IS (1918), No. 4, pp. 167-172) .—This article gives a historical survey 



of bovine tuberculosis and the measures taken in different countries for its 



control, a description of the nature and distribution of the lesions of the disease, 



65162°— 18 7 



