188 EXPERIMENT STATION RECORD. 



closed with ;i layer of boiled parchment paper. As soon as the blood 

 is coagulated it is placed in a cool place or in ice water, taking- care to 

 guard against dust. After 24 to 48 hours the clear liquid separated 

 out is measured into sterilized tlasks by means of a pipette, and if it is 

 to be kept for some days a few drops of chloroform are added. Injec- 

 tions are made at the low T er part of the chest or on the side of the neck. 

 The liquid is distributed in the subcutaneous tissue by gentle pressure. 

 Every trace of swelling generally disappears in about an hour and the 

 horse may do ordinary work the following or within the same day. The 

 conclusions arrived at by the author are that the method furnishes a 

 valuable means for producing immunity from the disease for a time and 

 for stopping enzootic lung fever. The injections do not, however, 

 always prevent the appearance of the disease when the animal is 

 already infected. We are not able at the present time to give an 

 accurate explanation of the action of serum injections in different dis- 

 eases, but, as regards the lung diseases, we can safely say that the 

 injections only increase or assist the natural power of resistance, and 

 it is therefore also probable that the effect may be abrogated when the 

 causal conditions of the disease appear especially strong in individual 

 cases. 



Properly conducted, the method is harmless. Nearly 1,000 injections 

 wero made in Denmark and in no case was there an appearance of 

 phlegm, fever, or similar effects. — f. W. woll. 



Texas fever, -T. W. Oonaway (Missouri tita. Bui. 37, pp. 81-139, Jigs. 

 11). — During the year cooperative experiments were carried on at the 

 Missouri station grounds in cooperation with the State Board of Agri- 

 culture and the Texas Experiment Station. The results are considered 

 in this bulletin under 4 heads: 



(1) Confirmation of the tick theory as to the transmission of Texas 

 fever. — Ticks were obtained from Texas; microscopical examinations of 

 the blood of animals were made; native cattle were exposed in the pres- 

 ence of southern native cattle; native cattle were exposed to southern 

 cattle from which the ticks had been removed ; the pasture was infested 

 with ticks taken from southern cattle; young ticks hatched artificially 

 from eggs laid by adult ticks picked from southern cattle were placed 

 on native cattle; experiments were made to show that the disease is 

 not transmissible by the excretions of southern cattle, and all with the 

 result of confirming the discovery of F. L. Kilborue, namely, that 

 infection is caused by these ticks. The method of rearing the ticks in 

 an improvised brood chamber formed by a Mason jar containing moist 

 earth and grass and several autopsies are described. 



(2) Experiments on the prevention of Texas fever. — The method of 

 dipping, in which the cattle are driven into a large vat filled with 

 water covered with oil, is described. Several different parasiticides 

 were employed, but that which gave the most favorable results was 

 cotton-seed oil. Some trouble was experienced with mixtures contain- 



