518 



EXPERIMENT STATION RECORD. 



which we propose the name vignin. Its composition, ;is found by the average of 

 closely agreeing analyses of nine fractional precipitates, is as follows: 



I "u/nin. 



Per cent. 



Carbon 52. 64 



Hydrogen 6. 95 



Nitrogen 17. 25 



Sulphur 50 



Oxygen 22. 66 



100. 00 

 "(2) Besides vignin, the cowpea contains a globulin which has the composition 

 and, so far as could be determined, the properties of phaseolin, which we have 

 found in the kidney bean (Phaseolus vulgaris), and the adzuki bean (Phaseolus 

 radiatus). ... 



"The composition of phaseolin as obtained from different seeds is shown by the 

 following statement: 



Phaseolin from different legumes. 



Cowpea. KMney Adzuki 



Carbon 



II \ tin i u en . 

 Nitrogen. . 

 Sulphur... 

 Oxygen . . . 



Per cent. 



52. 27 



6.97 



16.69 



.50 



23.57 



bean. 



Per cent. 



52. 58 



6.84 



1G. 47 



.56 



23.55 



Per cent. 

 52. 56 

 6.97 

 16.45 

 . 57 

 23.45 



100.00 [ 100.00 



"(3) The cowpea contains a third globulin, extremely soluble in very dilute salt 

 solutions, which could be precipitated but partially by dialysis in water and com- 

 pletely only in the coagulated form by dialysis in alcohol. This substance closely 

 resembles in properties and composition bodies obtained from several other legu- 

 minous seeds. Its composition, as found by analysis of 2 precipitates, one obtained 

 by dialysis in water and the other by further dialysis in alcohol, is as follows:" 



Per cent. 



Carbon 53. 25 



Hydrogen 7. 07 



Nitrogen 16. 36 



Sulphur 1.11 



Oxygen 22. 21 



100. 00 



Proteid of the white-podded Adzuki bean, T. B. Osborne aud 

 G.F.Campbell (Connecticut State Sta. Bpt. 1896, pp. 387-300).— The 

 object was to determine the nature of the globulin forming the chief 

 proteid constituent of the bean, hence the other proteid substances 

 occurring in small quantity were not studied. The analyses of 5 

 preparations showed the globulin to be identical in composition with 

 phaseolin as obtained from the white bean, 1 and the reactions were 

 found to be the same. In the solution from which the phaseolin had 

 been removed from the extract with ammonium sulphate a second pre 

 cipitate was obtaiued by saturation with ammonium sulphate, which 

 was purified and analyzed, and was found to be similar in composition 



'Connecticut State Sta. Rpt. 1893 (E. S. R,, 5, p. 1080). 



