r)S4 EXPERIMENT STATION RECORI). 



most cases Ix'iiiL;' lactic acid ornanisius, the rujuctiers and alivaiiiic 

 an'l otiicr species, and most of the acid specicvs not hictic. I)(>ing 

 crowded out. 



Fui'th(M' P^videnco of the Apparent Idontitv of Badihis coli and 

 Certain Lactic Acid Bacilli was present(^d by S. C. Prescott. The 

 authoi- prepai-ed a series of cultures of l)acteria corresponding- to lactic 

 acid bacteria isolated from grains and dairy products, and another 

 series of cultures of bacteria corresponding to the coli bacillus isolated 

 from fecal matter and from sewage polluted water. The two series 

 were compared as regards cultural reactions, morphological characters, 

 fermentative power, and pathogenic properties, and were found to be 

 practically identical in these respects. The bacteria from both sources 

 were shown to be somewhat pathogenic to guinea pigs. The general 

 conclusion was drawn that the two classes of bacteria studied were 

 actually identical. Bacteria corresponding to Bacillus coli are not 

 confined to the intestinal tract. The mere presence of such bacteria 

 in water does not, therefore, necessarily prove sewage pollution. 

 Their presence in large quantities would be more indicative of such 

 condition. 



L. A. Rogers reported the isolation of a fat-splitting torula yeast 

 from several sami)les of canned butter. The cells are elliptical, about 

 3.5 yu long and show little tendenc}' to form chains or bunches. The 

 torula shows a much weaker action than that of fat-splitting molds. 

 The acid number of a pure butter fat inoculated with a milk culture of 

 the torula increased in 2 weeks from 0.570 to 3.474. It ferments mal- 

 tose slowly at 37'^ C, but does not ferment lactose, galactose, levulose, 

 mannose, or sucrose. 



A Preliminary Chemical Study of Various Tubercle Bacilli was 

 reported by E. A. de Schweinitz. Analyses of tubercle bacilli, men- 

 tioned elsewhere, were reported, and the relation of human and bovine 

 tuberculosis was discussed. Investigations were reported which led 

 the speaker to the opinion that the two diseases are due to the same 

 organism. Tuberculosis was produced experimentally in a calf by 

 inoculation with a «'ulture of the tubercle bacilli from the organs of a 

 child which died of the disease, and the bacillus isolated from the 

 organs of the calf resembled the bovine more than the human form. 

 Experiments with monkeys showed them to be very susceptible to the 

 bovine tubercle bacillus. 



A paper })y M. Dorset on Egg Medium for the Cultivation of Tuber- 

 cle Bacilli was presented in substance by E. A. de Schweinitz. This 

 medium is easily prepared from fresh eggs. The tubercle bacillus 

 grows very readily upon it, colonies appearing in 6 to 7 days after 

 inoculation. (See p. 611.) 



Other papers of rather less interest in this connection were: Contri- 

 bution to the Study of Agglutinins, by W. W. Ford; The Reaction of 



