VKTEEINARY SCIENCE AND PRACTICE. 697 



guinea pigs mid ra'oliits it was found that an animal could be immunized against one 

 pathogenic organism and could then be innnunized against another organism without 

 losing its immunity toward the lirst species. It M'as found that a partial immunity 

 produced by one inoculation could be considerabry increased by sul)sequent inocula- 

 tions. In one experimental animal a marked agglutinating power was gradually 

 developed against 3 micro-organisms, the ty))lioid bacillus, Baderinin coli corinnune, and 

 th(! pyocyaneus organism. 



Immunization experiments -with Kraus' bacillus of rabbit influenza, E. 

 Jacobitz [Cnilhl Bait. u. Par., 1. Aht., .L' (1902), No. 4, Orig., pp. JSS, ..'S9).—\n 

 outbreak of intiuenza occurred among the rabbits in the laboratory in which the 

 author was working. Attempts were made to check the disease by different methods 

 of immunization. Rabbits were first treated with killed cultures, the experimental 

 animals receiving gradually larger doses of this material. The effect of the treatment 

 was nil, and no trace of immunity was to be observed. An attempt was also made 

 to protect rabbits against disease by increasing doses of living liacilli in very dilute 

 solutions. The results of these experiments were also negative. All attempts on 

 the iiart of the author to produce artificial immunization indicate that this result is 

 impossible. 



The effect of glycerin on virus, V. Galtier {Jour. Med. TYV. d ZootecJi., 6. scr., 6 

 (190;?), Fill., jip. 6-'>-7 i). — Since glycerin is ased so extensively in the preservation 

 of virus of different sorts, the author undertook experiments to determine the length 

 of time during whicli virus could be preserved in this reagent without losing its viru- 

 lence. The experiments included tests with antlirax, glanders, and tubercle bacilli. 

 It was found that fresh anthrax virus prepared from the pulp of affected organs 

 rapidly lost its virulence when preserved in pure glycerin. At the end of 55 hours 

 such material was attenuated to such an extent that it no longer produced infection 

 in guinea pigs or rabbits, even when inoculated in large doses. When affected 

 kidneys and spleens were preserved entire or in large pieces without ti'itui'ation in pure 

 glycerin the virulenee of the bacillus was attenuated much more slowly. During 

 the exi)eriments it was found tliat anthrax virus containing sjwres, as compared with 

 non-spore-bearing anthrax bacilli, resisted the action of glycerin much longer. The 

 virulence of glanderous material was retained in glycerin for a period of 10 to 12 

 days, or in some instances 17 or 18 days. It is believed, therefore, that glanderous 

 and tuberculous material or tissues suspected of being infected with these diseases 

 may be safely preserved for a few days in glycerin, until opp<irtunity is had for a 

 bacteriological or inoculation test. 



Aseptic metallic powder, L. Hoffmann {Berlin. ThicrdrzU. WchmcJir., 1902, 

 j\"o.s. 1, pp. 1-4; 7, pp. 109, 110). — The author conducted a number of experiments 

 with 2 kinds of powder ol)tained from tlie establishment of a metal beater. It was 

 learned by inquiry that these finely granulated powders, one of which was silver 

 and the other golden in color, exercised no injurious effects upon the skin of man or 

 animal. Experiments were therefore instituted for the purpose of testing the value 

 of these powders in the treatment of wounds. During these experiments quite sat- 

 isfactory results were ol)tained. It was found that the wounds could be directly 

 covered with either of the i)owders, and that little subsequent dressing or treatment 

 was re(jnired. 



The action of aseptic metallic powders, Schmidt {Berlin. Thienirztl. Wdtnxchr., 

 1902, No. 3, p. 39). — The author takes issue with Hoffmann concerning the action of 

 metallic powder on wounds. Hoffmann l)elieved that the powders exercised merely 

 a mechanical effect upon the wounds, preventing an infection fromtlie outside, while 

 the present author maintains that the aseptic action of metallic powders must be 

 ascribed partly to their chemical properties as well as to their physical properties. 



The therapeutic application of formaldehyde in a fixed form, F. Glage 

 {Dent. Thierdrztl. Wehmeln:, 10 {1902), No. 14, pp. 133, i.?^^).— The author found that 



