704 EXPERIMENT STATION RECORD. 



Serum diagnosis of glanders, A. Rabieaux [Rec. Med. VH., Paris, 8. .scr., 9 

 {190-2), No. 12, pp. 303-S09). — In testing the vahu' of serum reaction in the diagnosis 

 of glanders the author recommends the following technical method: The serum to 

 be examined was collected in as pure a condition as possible, and was then diluted 

 in sterilized distilled water in proportions varying from 1 : 10 to 1 : 1,500. The diluted 

 serum was then mixed with equal parts of a pure culture of glanders bacillus in pep- 

 tonized or glycerinated bouillon. The mixture thus obtained was maintained r.t a 

 temperature of 35 to 37° C, and examined at irregular intervals under a microscope. 

 During these experiments it was found that at a temperature of 35 to 37° C. the 2 

 kinds of glanderous and nonglanderous serum possessed (]uite different agglutinating 

 properties. When diluted in the proportion of 1:10 to 1 :50, the difference in tlie 

 agglutinating power of the 2 kinds of serum are quite manifest. Tvower temperatures 

 were found to have the effect of diminisliing the rapidity and intensity of the agglu- 

 tinating process. From the author's experiments it is reported that when a serum 

 manifests a well-marked agglutinating power in a dilution of 1 : 1,000, the animal from 

 which the serum was obtained should be considered glanderous. The method of 

 serum diagnosis is essentially a laboratory method, and is not recommended as repla- 

 cing other experimental methods which may be more convenient and satisfactory 

 under certain circumstances. 



A peculiar case of glanders in a horse in German Southwest Africa, Ekk- 

 MANand Kasewi-rm {Arch. IT/.s.s. v. ]>mkf. Tliicrh.,2S (1902), No. 1-2, ]>p. l//2-ir>2).—A 

 detailed description is given of a typical case of glanders in a horse, the usual symji- 

 toms of this disease l)eing W'ell developed. Not only did t he clinical symptoms clearly 

 indicate a case of glanders, but this diagnosis was strongly confirmed l)y a post- 

 mortem examination. The altered lymphatic glands were found to contain large 

 numbers of bacilli which agreed in all respects with the typical form of glanders 

 bacillus. Pure cultures of these organisms showed the usual behavior of the glan- 

 ders bacillus. However, in order to make the diagnosis more certain, inoculation 

 experiments were made on guinea i^igs, rabbits, and on the ass. All these experi- 

 ments gave negative results. After the experimental animals had been inoculated 

 for a sufficient length of time they were tested with mallein without showing a reac- 

 tion, and were, later, killed and carefully examined i)ost-mortem without finding any 

 evidence of the development of glanders. The authors believe, nevertheless, that 

 the original case was one of glanders, and by comparing the symptoms' of the dis- 

 ease and the behavior of the bacilli, it appears that the possibility of the case having 

 been pseudo-glanders is excluded. 



An epizootic of herpes among young horses, Fkoehner (Deut. Thierarztl. 

 Wdmschr., 10 {1902), No. 1, pp. 1-3, fig. 1). — The author described an extensive out- 

 break of this disease with special reference to the susceiitibility of various races and 

 colors of horses to infection. As a rule, the disease was for the most part confined to 

 the shoulder. The parts which were next affected were the neck, flanks, and head. 

 It was noticed that those parts of the skin which were i:)rotected by the long hair of 

 the mane and tail were never affected, and it is suggested that the fungi which pro- 

 duce the disease may not be able to penetrate to the skin through long hair, or that 

 the follicles of the long hair are more deeply located in the skin than those of the 

 ordinary short body hair. Notes are given on a large number of remedies which 

 have been recommended for the treatment of this disease, the most of which proved 

 to be of little value. It is maintained that after infection has once taken place at a 

 given point of the skin, epilation can not be prevented. Treatment should be 

 restricted therefore to attempts to prevent the extension of the disease. Antiseptic 

 salves should l)e repeatedly applied to the parts of the skin immediately surrounding 

 the affected points. 



Partial report upon the investigation of horse sickness, H. Watkins-Pitch- 

 FOKD {Aqr. Jour, and Mm. Rec, 5 {1902), No. 13, pp. 5S>7--;;0i).— The author has been 



