224 EXPERIMENT STATION RKCoRD. 



Determination of phosphates in natural waters, A. T. Lincoln and P. Barker 

 (Jour. Amer. Chem. Soc, 26 ( 1904), No. 8, pp. 975-080). — Satisfactory tests on water 

 of a modification of Schreiner's method ( E. S. R., 15, p. 444) in which a sufficient 

 amount of phosphate solution of known strength is added to make the proportion of 

 phosphoric acid approximately equal to that of silira are reported. 



Are inorganic phosphates found in seeds and seedlings? E. Schulze and 

 N. Castoro (Ztschr. Physiol, ('hem., 41 {1904), No. 5, pp. 477-484) .—From studies of 

 a numher of sorts of seeds the conclusion was reached that the phosphorus compounds 

 designed as a reserve material occur entirely or almost entirely in organic forms. 



If the seedlings grow for a considerable time without light it appears that a part of 

 the organic phosphorus is changed into inorganic forms. This change probably takes 

 place to a very limited extent in the case of germination under normal conditions. 

 In such cases lecithin should be mentioned as one of the compounds formed from 

 phosphorus reserve materials. The phosphoric acid occurs in the same part of the 

 seeds as the reserve protein, fat, and carbohydrates, and the fact that it increases or 

 decreases with the reserve protein is regarded as more than accidental. 



Potassium tetroxalate as a titrating reagent, O. Kuhling (Chem. Ztg., 28 

 (1904), Nos. 50, p. 596; 51, p. 612). — A reply to G. Lunge's criticism of the author's 

 1 1 ict hod of preparing this substance (E. S. R., 15, p. 649). 



The use of potassium tetroxalate as a titrating reagent, G. Lunge (Chem. 

 Ztg., 28 (1904), No. 60, pp. 701, 702). — A reply to criticisms by Kuhling (noted above). 



Potassium tetroxalate as a titrating reagent, O. Kuhling (Chem. Ztg., 28 

 (1904), No. 64, p. 752). — A reply to Lunge's article noted above. 



Dumas' method of determining nitrogen, A. Landsiedl ( Chem. Ztg., 28 (1904 ), 

 pp. 648, 644; abs. in Chem. Cenibl, 1904, II, No. 4, pp- 363,864, fig. 1).— An improved 

 measuring tube for use in this method is described. 



The volumetric determination of nitric nitrogen by means of ferrous 

 sulphate, G. Bailhache (Bui. Soc. Chirn. Paris, 3. ser., 31 (1904), No. 14, pp. 848- 

 846). — The author describes his modification of the Pelouze method substituting 

 ferrous sulphate for ferrous chlorid, operating in an atmosphere of carbon dioxid 

 and titrating the unoxidized ferrous salt with potassium permanganate or bichromate. 

 The author objects to Debourdeaux's modification of his method using a carbon 

 dioxid apparatus and a refrigerator (E. S. R., 15, p. 747) as difficult of operation. 



The estimation of ammonia in animal juices and organs, M. Nencki and 

 J. Zalessky (Arch. Sci. Biol. [St. Petersburg], 9 (1901), pp. 322-336; Chem. Cenibl., 

 1903, /, pp. 601, 602; abs. in Ztschr. Untersuch. Nahr. u. Genussmtl., 7 (1904), No. '■». 

 p. 554). — A modification of the usual method is described. 



The form in which nitrogen occurs in the proteid molecule, T. GBmbel 

 ( BeUr. Chem. Physiol, u. Pathol, 5 (1904), No. 5-6, pp. 297-312).— A critical study 

 of W. Hausmann's« method of determining the amount of different kinds of nitrogen 

 in protein is reported. This method depends on the cleavage of the protein with 

 boiling concentrated hydrochloric acid; the estimation of the amid nitrogen by dis- 

 tillation with magnesia; the separation of the diamin or basic nitrogen by precipita- 

 tion with phosphotungstic acid, and the estimation of the monamino nitrogen not 

 separated by the magnesia and phosphotungstic acid. 



The conclusion was reached that in the case of the amid nitrogen the results are 

 very accurate, and that they are quite accurate in the case of the monamino nitro- 

 gen, while in the case of the diamino nitrogen they are accurate within 0.8 of a per 

 cent, the values being generally too low. 



Concerning the form in which nitrogen occurs in albumin, C. Rothera 

 (Beiir. Chem. Physiol, u. Pathol, 5 (1904), No. 9, pp. 442-448).— Continuing work 

 referred to above, Hausmann's method was further studied. The conclusion was 



"Ztschr. Physiol. Chem., 27, p. 91; 29, p. 136. 



