408 MONTGOMERY. [Vol. XV. 



present, though variable in size and nun>ber. They do not 

 regularly arise at one particular part of the nucleolus, as we 

 found to be the case in the preceding species. Further, there 

 is rarely in this species a single large excentric vacuole ; but 

 as the figures show, usually a number are present, either 

 arranged in a circular row near the periphery, or in a row 

 around a larger central vacuole, or grouped together at one 

 point in the nucleolus. There can be no doubt that the larger 

 vacuoles are produced by the fusion of smaller ones, since two 

 or three smaller ones are frequently found in close contact 

 with each other. 



The double stain, haematoxylin and alum carmine, gives 

 different results from the preceding stains, in that by it not 

 only the different nucleoli within a nucleus become colored 

 differently, but also in some cases different stains of the 

 different portions of the same nucleolus are attained (Figs. 

 22-25). I^ ^s only the larger nucleoli, those with regular con- 

 tours, which become differentially stained in this manner. In 

 such a large nucleolus a portion of its substance stains a deep 

 blue (haematoxylin), another portion or portions purplish or 

 reddish (alum carmine) ; the part stained blue is usually central 

 in position, and encircling it is a zone of red-stained substance. 

 In one case (Fig. 22) the two opposite poles of the nucleolus 

 were reddish, the intermediate part being a deep blue. The 

 medium-sized, irregular nucleoli always stain blue throughout, 

 the smaller ones usually red, but sometimes blue. This stain, 

 accordingly, shows that in this gregarine some of the larger 

 nucleoli are composed of two different substances similarly' as 

 we had found two substances in the preceding species, though 

 there by using the methylen-blue-brasilin stain. 



With all three staining methods employed, a mass of irregular 

 granules is present in each nucleus, which stain less intensely 

 than the nucleoli. In the smallest nuclei (Figs. 22-25) these 

 granules are more or less regularly distributed through the 

 nucleus, but in the larger ones (Figs. 28, 31-35) they com- 

 pose a dense mass around the nucleoli or around the largest 

 nucleolus, while the peripheral portion of the nucleus remains 

 nearly free of them. Delicate, faintly stained fibers transverse 



