90 EXPERIMENT STATION RECORD. 



of diseased aiiiiiialH. Thorough (Usinfection has been insisted upon in all stables 

 where outbreaks of tlie disease were found. 



Phagocytosis during fatal infections, T. Tchistovitch {Ann. Imt. FaMeur, 

 14 (1900), Xu. 1^, pp. 80J-S1~'). — Rabbits were inoculated with fatal doses of strep- 

 tococcus and killed for examination after \, I, %, 1, 2, 4, and 6 hours. These experi- 

 ments showed that immediately after intravenous injection of streptococcus these 

 organisms are found in considerable numbers in the lungs, free at first and later 

 surrounded by polynuclear leucocytes. The leucocytes are numerous and invade 

 the capillaries. Phagocytosis in the lungs is by no means complete, and free strep- 

 tococci were found in all of the rabbits. The process of phagocytosis was more 

 complete in the lungs than in the liver. 



The hot fly (Gastrophilus equi), W. W. Froggatt [Agr. Gaz. New South Wales, 

 11 {IVOO), No. 11, pp. 947-951,1)1. 1). — The author gives a brief account of the life 

 history and habits of the more common species of this family which infest the horse, 

 ox, sheep, and camel. An experiment was conducted by T. 8ti'el in treating mules 

 for hot flies. After a preliminary feeding with bran mash, li drams of carbon bisul- 

 phid were given in a jjint of water, and the dose was repeated 3 hours later. In the 

 evening of the same day J pt. of linseed oil was administered. The treatment had 

 no apparent effect on the bot flies, although a large number of parasitic worms 

 were expelled, but it was not certain that the bot flies had not left the mule when 

 the treatment was administered. The species of bot fly which is common in 

 Victoria is said to lie (i. sdlutaria. 



A ne\7 process in the culture of tetanus bacillus, L. Debrand {Ann. Inst. 

 Pasteur, 14 {1900), No. 11, pp. 757-768). — The tetanus bacillus when cultivated alone 

 is anaerobic, and the experimenter has always the extra trouble of producing a 

 vacuum in order to secure abundant supplies of pure cultures. In experiments con- 

 ducted by the author it was found that when the tetanus bacillus was grown 

 together with Bacillus suhtilis, it vegetated rapidly in the presence of oxygen, and a 

 toxin w^as obtained from such cultures which possessed the same properties as the 

 toxin from the tetanus V)acillus grown alone under anaerobic conditions. This gives 

 an easy and convenient method for obtaining pure cultures of the tetanus bacillus. 

 It was also found that other bacteria beside B. suhtilis had the same effect on the 

 tetanus bacillus. Among these, mention may be made of B. mesetitericus and 

 B. anth7-acis. When grown with the anthrax bacillus, however, the tetanus bacillus 

 produces a very weak toxin. 



On the ringworm infection in man and animals, J. L. Bunch {British Med. 

 Jour., 1901, No. 2093, Epit., pp. 323-326, pis. 2, figs. ^).— The author discusses a 

 nundier of cases of ringworm which were communicated from horses and cats to 

 man. The nature of the fungus parasite which was isolated is described in deta,il, 

 together with remarks on its behavior in different culture media. 



Agglutination of bacillus of Koch by tuberculous discharges, P. Courmont 

 {Arch. Med. E.vper. et Anat. Path., Paris, 1. ser., 12 {1900), No. 6, pp. 697-732). — The 

 author's experiments included series of tests with serous fluids and blood serum from 

 man and experimental animals. When animals were inoculated with the virulent 

 form of tuberculosis the serum reaction was almost always negative, although death 

 followed later in every case. On the other hand, when the animals were inoculated 

 with a less virulent form of tuberculosis the serum reaction was always positive, in 

 the proportion of 1 to 20 in the case of the guinea pig and 1 to 600 in the case of the 

 dog. Experiments with various serous discharges which were not tuberculous gave 

 reactions which were always negative. 



The general results obtained may be stated as follows: Non tuberculous, serous 

 fluids do not agglutinate the tubercle bacillus under ordinary conditions in the propor- 

 tion of 1 to 5. The majority of tuberculous, serous fluids show an agglutinating power 

 in proportions of from 1 to 5 and 1 to 20. A certain proportion, however, of tuber- 



