VETERINARY SCIENCE AND PRACTICE. 



389 



In anotlier experinu'iit in wliich 1 gni. of trypsin was usfd in 100 liters (jf milk the 

 cheese made did not exliil)it any difference from the cnntrol. It ajtpears that so 

 email an amomit of trypsin in tlie eheese is without infinencein the ripening, while 

 25gm. i)er 100 liters, as in the jjrevious experiment, is too much, causing a bitter taste. 



It was found that with cheese heated only slightly the addition of pepsin hastened 

 the ripening, as pointed out by Bal)cock and Russell. The following table shows the 

 results after -4 months with skim-milk clieese heated to only 3S° C: 



Influence of pcjixni In ripeiihuj du'e-fe. 



A number of speculations are made, btit the results are not of sufficient number or 

 uniformity to draw definite conclusions. The most marked indication is that of the 

 much more intense action of trypsin over that of galactase and pepsin. 



Assistance offered to cheese factories and creameries in Ontario, F. C. 



Hakkisox [Ontario A(jr. CoL and Ejpt. Form Jijit. 1900, jiji. 7"-7J). — Brief notes are 

 given on a number of troubles in cheese factories and creameries due to bacterial 

 infection, and assistance in investigating any such troubles in the Province is offered 

 by the bacteriological dei)artment. 



VETERINARY SCIENCE AND PRACTICE. 



Tlie lymph after intravenous injection of tetanus toxin and tetanus anti- 

 toxin, F. Raxsox {Ztxrhr. Physiol. Chem., 29 {1900), Xo. 4-3, pp. 349-372).— The 

 chief ol^ject of the author's investigation was to determine the agency of lymph in 

 distributing tetanus toxin and tetanus antitoxin throitghotit the organism. As 

 experimental animals dogs were chosen, since the thoracic dtict could be readily 

 exposed in these animals and the lymph allowed to flow out into receptacles 

 through a canula. Before being operated upon the dogs were treated with mor- 

 phin and antesthetized. They were kept in this condition during the experimental 

 period. The lymph was received in glass vessels from the thoracic duct, and the 

 length of time after inoculation was noted. The blood which was studied was taken 

 from the femoral artery. For determining the strength of toxin and antitoxin as 

 found in the lymph or blood, inoculation tests were made with mice. The results of 

 the author's experiments may be summarized as follows: After the tetanus toxin is 

 injected into the blood circulation, a considerable portion of it rapidly passes into 

 the lympli. If the 1)lood and lymph systems are left intact, the toxin is found 

 equally distributed in the blood and lymph 26 hours after injection. If the thoracic 

 duct is opened shortly before the intravenous injection, the toxin content of the 

 blood remains considerably greater than that of the lymph for 6 hours after injec- 

 tion. After injection of tetanus antitoxin (horse serum) into the blood, this sub- 

 stance rapidly finds its way into the lymph. If the thoracic duct is opened, the 

 blood contains more antitoxin than tiie lymph for a period of at least 6 hours. If 

 the thoracic duct is intact, the antitoxin does not become equally distributed in the 

 blood and lymph after a period of (>S hours, the blood containing during this time 

 consideral)ly more antitoxin than the lymph. Xo noteworthy changes take place in 

 10409— No. -i 7 



