792 EXPEEIMENT STATION RECORD. 



A study of the larvae of Gastrophilus in the stomach of the horse, J. Guyot 



{Arcli. Parasit., 4 {1901), Xo. 2, jjp. 169-221, figs. 11).— X review is given of the 

 literature of the subject in connection with a bibliography of the more important 

 articles relating to Gastrophilus. A historical discussion is presented on Gastrophilus 

 intestinalis, G. hsemorrhoidalis, and G. nasalis. Special attention is devoted to G. intes- 

 tinalis, which is considered by the author to be a more correct name than G. equi for 

 the common species which infests the stomach of the horse. The eggs in different 

 stages of the larvpe are described in detail and considerable attention is devoted to 

 the manner in which the larvte are attached to the wall of the stomach, and to the 

 pathological lesions which their presence causes. Experiments were conducted for 

 the purpose of testing the action of certain chemicals on young larvw. It was found 

 that the larvae could live for 4 days in ordinary water, for 1 day in olive oil, and for 

 14 hours in corrosive sublimate in the proportion of 1 : 1,000. When placed in cer- 

 tain volatile substances such as bisulphid of carbon, benzin, ammonia, sulphuric ether, 

 and chloroform, the larvae died instantaneoush^ Experiments with full-grown larvse 

 showed that they could live 5 days nnmersed in a 3 per cent solution of formalin, 

 and for 1 hour in a saturated aqueous soluton of corrosive sublimate. It was found 

 that young larvae, which were hatched from eggs which had l)een removed from the 

 hair of the horse, were alive 3 months after the removal of the eggs. The patho- 

 logical lesions caused by the botflies in the stomach of the horse are restricted to the 

 mucous layer of the stomach which is destroyed at the point where the botflies are 

 attached. An accumulation of leucocytes at such jsoints is also noted. 



Rabies, ]M. P. Ravexel {Pennsylrania Dept. Agr. Bui. 79, pp. 28, figs. 3). — A general 

 discussion of this disease is presented, including its history and distribution; a list of 

 animals subject to the disease; the cause of the disease; nature of the virus; method 

 of invasion; resisting power of the virus; danger from bites of ral)id animals; period 

 of incubation; influence of seasons; the various types of the disease in man, dog, cat, 

 horse, cow, and birds; pathological changes in the tissues of animals attacked by the 

 disease, and the various features of the Pasteur method of preparation of vaccine; 

 and treatment of affecteil animals. 



The action of leucotoxic serum on lesions in the central nervous system 

 in cases of rabies, C. Fr.\xca iCompt. Rend. Soc. Biol. Paris, 53 (1901), Xo. 17, pp. 

 502-504). — Exjieriments previously conducted with rabies indicated that death was 

 partly due to an excessive formation of leucocytes during the acute stages of the dis- 

 ease. It was decided, therefore, to try experiments for the purpose of testing this 

 theory. Dogs were experimentally inoculated with rabies and after the appearance 

 of the acute symptoms were treated with injections of the leucotoxic serum. Con- 

 siderable improvement was noted, and another injection gave evidence of increased 

 benefit from this treatment. Further work- along this line is promised, and the 

 author believes that toxins may ultimately be produced which may be used for the 

 i:)urpose of preventing too pronnunced development of leucocytes. 



Negative chemotaxis of the leucocytes of rabbits which have been inocu- 

 lated -with a pure culture of the bacilli of fo^wrl cholera, A. Zilberberg and J. 

 Zelioxy {Ann. Inst. Pasteur, 15 {1901), Xo. S, pp. 615-629, pi. ^).— The author briefly 

 reviews the literature on the subject and discusses in detail the methods and results 

 of his investigations. Since rabbits are especially susceptible to fowl cholera these 

 animals were considered most suitable for investigation on the subject of negative 

 chemotaxis. The experimental animals were inoculated hypodermically and in the 

 body cavity with pure cultures from artificial nutrient media and from natural fluids 

 of the body. The animals were killed within from 1 to 5 hours after inoculation and 

 various organs, including the liver, kidneys, spleen, spinal column, and lungs, were 

 examined for the purpose of determining the relation between the pathogenic Imcteria 

 and the leucocytes. After a hypodermic or intraperitoneal injection of a virulent 

 culture of fowl-cholera bacilli from gelatin, the complete absence of the phagocy- 



