BOTANY. 



605 



teria from the tubercles of Rohini a and Fisuni showed different effects 

 on the pUmts, but from this difference the authors think there was 

 nothing to show that the bacteria were specifically distinct. Recently 

 several series of experiments were conducted in pure quartz sand with 

 inoculations from pure cultures of bacteria from various legumes. In 

 one series peas and the common locust were inoculated with cultures 

 from the tubercles of Pisum sativum, Vicia sejmim, Medicago satii-a, 

 Robin ia pseudacacia, and Caraganaarhorescens. Tubercles were formed 

 on the peas from all the inoculations, while on the locust only those 

 receiving the cultures from the tubercles of Rob inia and Caragana pro- 

 duced any. In another series Latliyrus latifolius was inoculated from 

 pure cultures of Fisum, Vicia, and Robinia bacteria. Only the first two 

 produced tubercles. In the third series idants of Robinia pseudacacia, 

 Acacia loplmniha, Vicia villosa, and Pisum sativum were inoculated from 

 cultures of 2-year-old tubercles of Robinia, 2 year-old tubercles of Cara- 

 gana, tubercles of Vicia and Pisum. The tabulated results observed 

 are shown below. Before the experiments were completed a fungus 

 destroyed some of the x)eas and they were necessarily omitted from the 

 last two tables. 



Transpiration from inoculation to harvest. 



Inoculated witli pure cultures from — 



Robinia. Acacia. 



Gc. 



Robinia pneudacacia ' 3, 570 



Acacia lophantha 1. 538 



Vicia villosa , 9:U 



Fisum sativum 1, 380 



Cc. 

 1,136 

 3, 805 

 1,097 

 1,034 



Tida. 



Cc. 



1, 425 

 1,205 

 4,978 

 1,265 



Pisum. 



Cc. 

 1,396 

 1,511 

 1, 277 

 1,849 



Average height of plants at harvest. 



Chemical analysis of plants. 



Robinia dry substance grama . 



Rubinia nitrogen milligrams. 



Acacia dry substance grams. 



Acacia nitrogen milligrams. 



Vicia dry substance grams . 



Vicia nitrogen milligrams . 



Inoculated with pure cultures from- 



Robinia. Acaeia. 



7.402 



232. 100 



1. 953 



17. 000 



.783 



12. 900 



1.158 



16. 600 



6.943 



109. 800 



.866 



14. 700 



Vicia. Pisum. 



0.858 

 13. 500 



1.248 

 16. 200 



9.133 

 264. 000 



1.479 

 21. 100 



1.817 

 19. 700 



1. 033 

 22. 600 



From the above tables it will be seen that in all but one case each 

 plant was most favorably affected when it was inoculated with bacteria 

 from the tubercles from its own species. 



