105 



rabbits from tbis at sbort intervals. Tboc cxix'riineiits indicato tbat 

 the virus lost its vitality in from one to four months. 



Eft'ect of (lisinfeetants on the rims of liog clwlem. — A large number 

 of experiments are recorded. The method was to add a few (Iroi)s of 

 euhurc liquid containing the germs to the disinfectant solution in a 

 sterilized watch-glass under a bell glass. After certain regular inter- 

 A-als of time platinum loops holding about one one-hundredth cubic 

 centimeter were used to transfer this li(|uid to tubes of beef infusion. 

 These were then placed in a thermostat and watched for a number of 

 days; if no growth resulted, the bacilli Avere considered to have l)een 

 destroyed. Ecsults Avere briefly as follows: A saturated solution of 

 connnon salt destroyed the bacilli in from twenty-one to twenty-nine 

 days: mercuric chloride. 1 to 75.000, in seven days: carholic acid, 1 

 to 80, in five minutes: iodine water in fifteen minutes; 'perrnanga- 

 ■juite of potash. 1 to H.OOO. in fifteen minutes; mercuric iodide, 1 to 

 1,000,000, in ten minutes; sul/>Jtate of ropper. 1 to 200, in five min- 

 utes; 1 to 1,000. in twenty minutes: liijdrochloric acid, 1 to 500, in 

 five minutes; chloride of zinc, 1 to 10, in fifteen minutes; sulphuric 

 acid. 1 to 2,000, in ten minutes. A mixture of equal parts of crude 

 cat'hoUc acid and commercial sul pit uric acid. 1 to 200, destroyed the 

 bacilli in mixtures containing nnich organic matter in one hour. 

 Ordinary lime destroyed the bacilli in water when present in only 0.03 

 jjer cent: in beef infusion, when there was 0.08 per cent, and when 

 there Avas considerable albuminous matter in suspension it required 

 0.32 per cent. It is concluded from other experiments that from 0.75 

 per cent to 1 i)er cent of lime will destroy the hog cholera bacilli in 

 soil. 



Is there any spore state in the life liistor)/ of the l><u-ilhi>< of hog 

 cholera? — This (]uestion is c(msidered from vai'ious ])oints of vicAV, 

 and is decided in the negative. 



Ways in which swine hecome infected. — Experiments are detailed. 

 shoAving the infection of hogs by feeding on the viscera of infected 

 animals, and on pure cultures of hog-cholera bacillus, by subcutaneous 

 inoculation Avith blood from affected swine and Avith culture liquids, 

 and by intraA'enous inoculation. 



Ohserrations on the action, of hog-dodcra Ixictcria. — Under this 

 head an explanation is given of the ways in which the different symp- 

 toms and lesions are produced in this disease. 



Bacteriologiccd investif/ation of hog cholera in Xchruxl.'u. Ilf/nois. 

 and Maryland. — This contains details in regard to the isolation and 

 study of the hog-cholera germs from these different sections. While 

 some slight differences in the manner of groAvth of the germ Avere 

 observed, it was plain that they were all of the same species. 



Relation of hog cholera to the puUic health.— Tho hog-cholera ba- 

 cillus resembles in some respects the germ of typhoid fever, but the 

 conclusion is reachetl that the two diseases are entirely distinct. Ana- 



