DISCUSSION OF ZIRKLE'S AND TOBIAS' PAPERS 



389 



Fig. 1. Control preparation. There is rather uniform storing of acridine orange in 



the cell vacuole with fluorescence of various shades of yellow-orange. The cytoplasm 



along the cell walls fluoresces green; the nuclei are yellowish green. 



Fig. 2. Section has been iiiadiated for 4 min. Two "spot" areas have an intense 

 concentration of dye (brick-red fluorescence). This has migrated from adjacent 

 cells to the damaged cytoplasm. The remaining cells have the appearance of the 

 control, except for some clumping of dye along the cell walls in the cytoplasm. 

 Adjacent cells are frequently affected, and this is the cause for the double cell ap- 

 pearance. 



