276 



4. KüHNE's crystalviolet solution 2 minutes. 



5. Wash in 0.6 % NaCl solution. 



6. Dry with filterpaper. 



7. LUGOL's solution 2 minutes. 



8. Dry with filterpaper. 



9. Eosin solution in alcohol. 15 seconds. 



10. Dry with filterpaper. 



11. Alcoh. absol. 30 seconds. 



12. Xylol etc. 



The nuclea are blueish, the bacteria black, the rest is red. 



On comparing three animals, one septic, one immune, and 

 one normal, the differences in the sections are very great. In 

 all cases the original condition (I mean the condition immediately 

 after the treatment) presents the same aspect. Counting the 

 bacteria per endothelial cell I found from 1 — 10 bacteria per 

 cell, on the average. But when the bacteria have undergone 

 the influence of the endothelium for 8 hours, then the differences 

 become apparent. In the septicaemic animal the bacteria have 

 increased in number. They are lying in small groups on and 

 in the endothelial cells. After waiting longer, 16, or 24. hours, 

 the bacteria have gone on increasing and in many cases are 

 innumerable, still lying in and on the endothelial cells. Quite 

 otherwise are the developments in normal animals and in 

 animals which have been immunised. In the normal animals 

 the number of bacteria per cell for a long time remains stationary. 

 Then they start increasing, but slowly and not to any great 

 extent. This at least is the rule. But variations occur. In striking 

 contrast with these observations are the developments in the 

 immunised animals. Here the bacteria disappear, after having been 

 in contact with the endothelial cells for 24 hours. After 8 and 16 

 hours a decrease, or at last a retardation of growth is observed. 



These findings were of a constant nature, both in rabbits 

 and rats, with any sort of coccus. Of course the quantities of 

 bacteria showed differences, and the time in which the events 

 took place were different, but always these facts stood out 

 clearly, that bacteria could grow on endothelium of septic 

 animals, could maintain themselves to a certain degree on 

 endothelium of normal animals, but were killed or retarded in 

 growth on the endothelium of immunised animals. 



