BY R. GREIG-SMITH. 



153 



visionally named T.P. 1 and T.P.2, the former producing a white 

 colony, the latter an ivory-white on nutrient agar. T.P.I lique- 

 fies gelatine quickly and foims no slime on dextrose- media, while 

 Bac. T.P, 2 liquefies gelatine slowly, and forms a slime from 

 dextrose. The belief was raised by other experiments that the 

 formation of slime withdraws nutrients from the medium, and 

 yields a poor extract; but whether the toxin-formation has any- 

 thing to do with the production of slime, has yet to be deter- 

 mined. Bac. 'J\P.l, the slime-free ally of Bac. T.P.2, was tested 

 under the conditions of the last experiment, viz., in hay-infusion, 

 with and without dextrose, and at periods of 3, 6, and 10 days, 

 but no evidence of toxin-formation was obtained. 



An attempt was made to determine the effect of slime-forma- 

 tion upon the production of toxin by using a race of Rhizobium 

 from the soil, which formed a luxuriant slime on solid dextrose- 

 media. 



Experiment xii. 



A 13-days' test with hay-infusion and dextrose was found to 

 be sterile, and has been omitted. Although these tests were 

 intended to see the effect of slime-production upon toxin- forma- 

 tion, it happened that no slime was obtained on the porcelain 

 filter from the dextrose solution, and, but for the growths on 

 agar-slopes, one would have thought that the cultures were dead. 

 However, the slow reduction of the extract/ water ratio as time 

 went on, and the great increase obtained on boiling the dextrose- 

 solutions were again noted. 



