BY R. GREIG-SMITH. 



157 



130". When cold, lOc.c of a suspension of Bac. T.P.2 were 

 added to each portion. After various periods of incubation at 

 18**, a portion was transferred to a shaking bottle, treated with 

 500 c.c. of distilled water, and shaken 50 times at 10-minute 

 intervals for an hour. The suspension was then filtered through 

 paper and porcelain, and the usual procedure followed. 

 Experiment xiv.a. 



As the experiment did not seem to be going to give any useful 



information, the remaining portions were extracted with different 



quantities of water to test the influence of various strengths of 



extract. 



Experiment xiv.b. 



Growth of Bac. prodigiosus in extracts of leaf- 

 mould infected with Bac. T.P.2, and incubated 

 for 11 days at 18°. 



Extract, raw 

 Extract, raw, 80% 

 Extract, raw, 60% 

 Water-control 

 Extract/water ratio 



The results of Experiment xiv.a, are much the same as have 

 been obtained in solutions, viz., a lessening of the nutritive and 

 of the boiling effects, as the period of incubation proceeded. In 

 Experiment xiv.b, we have the dilution-effect becoming more pro- 

 nounced as the water used in making the extract became less, 



12 



