Pammel — Anatomical Characters of Seeds of Leguminosae. 137 



Malpighian. Cells 64.4 // in length. The cuticle forms a 

 continuous layer; light line close under the cuticle; cell-wall 

 between cuticle and light line differentiated into a clearer por- 

 tion. Cell-walls and cells nearly colorless. Cell-cavity filled 

 with small protein grains which on the addition of chlor-iodide 

 of zinc are colored brown ; the cell-wall colors blue. Several 

 canals project into the walls from the cuticularized layer. The 

 Malpighian cells are somewhat elongated in a surface view ; 

 canals extend from the central cavity and branch toward the 

 periphery. The branches number from six to seven. 



Osteosclerid . Cells as wide above as below, with large in- 

 tercellular spaces. The cell-walls are thickened and with 

 chlor-iodide of zinc color blue. The contents are granular 

 and color brown with iodine. 



Nutrient. Not strongly developed; consists of three or 

 four layers of strongly compressed cells. The cell-walls are 

 not greatly thickened. This layer contains the yellow pig- 

 ment and the fibro-vascular elements. Not infrequently the 

 layer is differentiated into two parts. 



Endosperm. This attains considerable size on the sides of 

 the seeds. It is divided into three parts. In the first or aleu- 

 rone layer, the cell-walls are greatly thickened ; the cells are 

 filled with fat and protein. The aleurone layer uniformly 

 surrounds the remainder of the endosperm. The second por- 

 tion consists of very thick-walled cells with large pore canals ; 

 this varies in width from a few to fifteen cells. The cells 

 contain aleurone grains and fat. On the addition of chloral 

 hydrate the fat collects in drops. The lower part of the 

 endosperm is made up of two or three rows of thick-walled 

 cells, much longer than broad, containing protein grains. The 

 cell-walls of the endosperm color blue with chlor-iodide of 

 zinc ; contents color brown with iodine. 



Embryo. The first row of cells forms a continuous layer. 

 The outer cell-walls are more strongly thickened than the 

 lateral. The remaining cells are thinner-walled and much 

 larger, irregular, with intercellular spaces. The cells are 

 filled with aleurone grains and fat, but no starch. Iodine 

 colors the aleurone grains brown ; the cell-walls are colored 

 blue with chlor-iodide of zinc. 



