BY DR. OSCAR KATZ. 516 



The following pages contain an account of my researches with 

 regard to the microbes of chicken-cholera. These researches, as 

 far as they were carried out before April last, were made the 

 subject of five Progress Reports laid before the Commission from 

 time to time, and printed in the Volume of Proceedings of that 

 Commission. 1 think that sufficient interest attaches to the sub- 

 ject to be dealt with in a scientific journal. For this purpose the 

 whole avciilable material, including that which was obtained since 

 April last, has been worked up and grouped in an appropriate 

 manner. 



To Messrs. F. Dillon Bell and J. P. Meagher, w^ho in succession 

 were Assistants on Rodd Island, I am indebted for the services 

 rendered by them in regard to the various experiments. 



General Remarks. 



The microbes with which all the experiments recorded in the 

 following pages were carried out, were descended from those which 

 were brought to Sydney from Paris by Pasteur's representatives. 

 When, August 4th, 1888, the latter concluded their experiments 

 of demonstration, which were begun about a month previously 

 (July 7th), and to which attention has already been directed in 

 the introduction (a special report on that demonstration may 

 be found in the Volume of Proceedings of the Royal Com- 

 mission), I took, with M. Loir's permission, some blood from the 

 heart of a rabbit which had died after feeding on virulent broth- 

 culture of the chicken-cholera microbes. Pure cultures were 

 obtained from a *' colony " on nutrient gelatine (after Esmarch's 

 roll-method) from the blood of a rabbit, which had been inoculated 

 with broth-culture in second generation, derived originally from the 

 above-mentioned sample of blood. 



In ray experiments, partly such material was used as originated 

 from that " colony," and was cultivated from tube to tube ; partly 

 cultures prepared directly from the heart-blood of rabbits newly 

 dead from virulent " chicken-cholera," and not otherwise diseased. 

 Such blooJ, as a rule, only contains the microbes under considera- 

 tion. 



