518 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



other micro-organisms, highly pathogenic for rabbits, e.g.^ anthrax 

 bacilli, are powerless."^ 



Pasteur states that the action of chicken-cholera microbes is 

 much more pronounced in the case of rabbits, than in that of 

 fowls.f My observations in this direction — my material for 

 experiments was derived from rabbits dead from " chicken- 

 cholera " — are thus far in accordance with Pasteur's statement. 



With regard to the effect of subcutaneous application of the 

 microbes on rabbits, I can assert that the result which I obtained 

 with material of U7idoubted full virulence (blood ; artificial cul- 

 ture) on wild rabbits (also one tame one), which had not been pre- 

 viously treated in any way — I estimate the number of wild rabbits 

 used in that way at about one hundred and fifty — was always a 

 positive one. All of them succumbed to a disease which owed its 

 origin to the chicken-cholera microbes. The time which it took 

 from inoculation to death diflered according to the degree of con- 

 'centration of the virulent object introduced, and according to the 

 individuality of the rabbits. Generally speaking, the microbes 

 thus administered kill speedily. Instances hereof may be found in 

 sufficient numbers later. The shortest time actuall}'- observed in 

 a full-grown healthy specimen (^), inoculated between the shoulder- 

 blades with 4^ ccm. (I minim) of heart-blood from a rabbit newly 

 dead from " chicken-cholera," was about 8J hours ; at another 

 time, in the case of a half-grown rabbit inoculated at the belly with 

 a small quantity of fresh broth-culture of the fourth genera- 

 tion, it was less than 7f hours. The longest space of time was 

 observed in a full-grown, but apparently young doe, namely 

 about forty-eight hours. This rabbit had been inoculated (at the 

 belly) with ^ ccm. of virulent rabbit-blood (see Table III., 

 Rabbit No. 34 ; also p. 552). 



*J. Straus et D. Sanchez-Toledo, •' Recherches microbiologiques sur 

 I'ut^rus apres la parturition physiologique." Annates de VInstitut Pasteur, 

 Tome II., No. 8, 1888, p 433. 



t Sur la destruction des lapins en Australie et dans la Nouvelle-Z^lande. 

 Annales de VInstitut Pasteur. Tome II., No. 1, 1888, pp. 5-6. 



